Salehi Zivar, Abadi Ali Saber Hossein, Ismail Patimah B T, Kqueen Cheah Yoke, Jelodar Mohammad Halimi, Kamalidehghan Behnam
Department of Biology, Faculty of Science, University of Guilan, Rasht, Iran.
Dig Dis Sci. 2009 Nov;54(11):2399-403. doi: 10.1007/s10620-008-0633-z. Epub 2008 Dec 12.
Helicobacter pylori is the major cause of active chronic gastritis and peptic ulcers in humans and has been linked to gastric carcinoma and lymphoma. The vacuolating cytotoxin vacA and cag pathogenicity island (cag PAI) are two identified virulence factors that are considered to have an important role in the pathogenesis of H. pylori infection. The aim of this study is to investigate the H. pylori vacA alleles in Iranian patients with peptic ulcer disease. In order to investigate this, biopsy specimens were obtained from 84 patients with gastric ulcer, gastritis, and duodenal ulcer. DNA extraction and PCR were used to detect the presence or absence of glmM, cagA and to assess the polymorphism of vacA. Of the 77 glmM PCR-positive biopsy specimens, 55 (71%) had the vacA signal sequence genotype s1, and 22 (29%) had subtype s2. vacA mid-region analysis revealed that 31 (40%) were vacA m1 and 46 (60%) were m2. The presence of the cagA gene correlated with vacA signal sequence type s1, whereas type s2 was predominantly found in cagA-negative samples (P < 0.001). Thus, the detection of vacA and cagA, virulence markers described in several clinical outcomes may be used to help the treatment and prevention of H. pylori in Iran.
幽门螺杆菌是人类活动性慢性胃炎和消化性溃疡的主要病因，并且与胃癌和淋巴瘤有关。空泡毒素VacA和细胞毒素相关基因致病性岛（cag PAI）是已确定的两种毒力因子，被认为在幽门螺杆菌感染的发病机制中起重要作用。本研究的目的是调查伊朗消化性溃疡病患者中的幽门螺杆菌VacA等位基因。为了进行此项调查，从84例胃溃疡、胃炎和十二指肠溃疡患者获取活检标本。采用DNA提取和聚合酶链反应（PCR）检测glmM、cagA的存在与否，并评估VacA的多态性。在77例glmM PCR阳性的活检标本中，55例（71%）具有VacA信号序列基因型s1，22例（29%）具有s2亚型。VacA中间区域分析显示，31例（40%）为VacA m1，46例（60%）为m2。cagA基因的存在与VacA信号序列类型s1相关，而s2型主要见于cagA阴性样本（P<0.001）。因此，检测VacA和cagA这两种在多种临床结局中描述的毒力标志物，可能有助于伊朗幽门螺杆菌的治疗和预防。
