White William N, Raj Ashok, Nguyen Mai-Dung, Bertolone Salvatore J, Sethu Palaniappan
Department of Bioengineering, University of Louisville, 2210 S. Brook Street, 357 Belknap Research Building, Louisville, KY 40202, USA.
Biomed Microdevices. 2009 Apr;11(2):477-83. doi: 10.1007/s10544-008-9253-9.
Nucleated cell populations, including leukocytes and circulating endothelial cells, provide an ideal sample for studies seeking to understand the pathogenesis of diseases for development of drugs and treatments. Conventional leukocyte enrichment protocols have limitations with respect to selective cell loss and artifactual activation. An automated microfluidic device was developed for leukocyte enrichment from peripheral blood to ensure enumeration of high quality sample without cell loss or artifactual activation. Pre-clinical trials have shown the efficiency of the device to maximize cell yield and minimize artifactual activation in comparison to conventional techniques. Clinical validation and the ability of the microfluidic technique to enrich leukocyte samples to understand disease processes was accomplished in this study by quantifying circulating nucleated cells and their activation status in healthy controls and mild phenotype sickle cell disease (SCD) patients. Results confirm the clinical effectiveness of this technique to accurately characterize immune and inflammatory status.
包括白细胞和循环内皮细胞在内的有核细胞群体，为旨在了解疾病发病机制以开发药物和治疗方法的研究提供了理想样本。传统的白细胞富集方案在选择性细胞损失和人为激活方面存在局限性。开发了一种用于从外周血中富集白细胞的自动化微流控装置，以确保获得无细胞损失或人为激活的高质量样本计数。临床前试验表明，与传统技术相比，该装置能够最大限度地提高细胞产量并最小化人为激活。本研究通过量化健康对照和轻度表型镰状细胞病（SCD）患者的循环有核细胞及其激活状态，完成了微流控技术富集白细胞样本以了解疾病过程的临床验证及其能力。结果证实了该技术在准确表征免疫和炎症状态方面的临床有效性。
