用于从全血中浓缩白细胞的微流控双水相系统。
Microfluidic aqueous two phase system for leukocyte concentration from whole blood.
作者信息
Soohoo Jeffrey R, Walker Glenn M
机构信息
The Joint Department of Biomedical Engineering, North Carolina State University and the University of North Carolina at Chapel Hill, Raleigh, NC 27695-7115, USA.
出版信息
Biomed Microdevices. 2009 Apr;11(2):323-9. doi: 10.1007/s10544-008-9238-8.
Leukocytes from a whole blood sample were concentrated using a microfluidic aqueous two phase system (microATPS). Whole blood was simultaneously exposed to polyethylene glycol (PEG) and dextran (Dex) phase streams and cells were partitioned based on their differential affinity for the streams. The laminar flow characteristic of microfluidic devices was used to create zero, one, and two stable interfaces between the polymer streams. Three different patterns of three polymer streams each were evaluated for their effectiveness in concentrating leukocytes: immiscible PEG-PEG-Dex, immiscible Dex-PEG-Dex, and miscible PEG-PBS-Dex. The most effective configuration was the Dex-PEG-Dex stream pattern which on average increased the ratio of leukocytes to erythrocytes by a factor of 9.13 over unconcentrated blood.
使用微流控水相两相系统（microATPS）对全血样本中的白细胞进行浓缩。全血同时暴露于聚乙二醇（PEG）和葡聚糖（Dex）相流中，细胞根据它们对这些流的不同亲和力进行分配。微流控装置的层流特性被用于在聚合物流之间创建零、一和两个稳定界面。评估了三种不同的由三种聚合物流组成的模式在浓缩白细胞方面的有效性：不互溶的PEG - PEG - Dex、不互溶的Dex - PEG - Dex以及互溶的PEG - PBS - Dex。最有效的配置是Dex - PEG - Dex流模式，与未浓缩的血液相比，其白细胞与红细胞的比例平均提高了9.13倍。
Zotero 插件