Bouhlel Ines, Kilani Soumaya, Skandrani Ines, Ben Amar Rebaii, Nefatti Aicha, Laporte François, Hininger-Favier Isabelle, Ghedira Kamel, Chekir-Ghedira Leila
Unité de Pharmacognosie/Biologie Moléculaire 99/UR/07-03. Faculté de Pharmacie de Monastir, Rue Avicenne 5000 Monastir, Tunisie.
Nutr Res. 2008 Mar;28(3):190-7. doi: 10.1016/j.nutres.2007.12.011.
Three extracts were prepared from the leaves of Acacia salicina: aqueous, methanol, and ethyl acetate extracts. The antigenotoxic properties of these extracts were investigated by assessing the inhibition of mutagenicity of the indirect-acting mutagen benzo[a]pyrene using the Ames assay and the genotoxicity of the direct-acting mutagen, hydrogen peroxide, using the "Comet assay." Aqueous, methanol, and ethyl acetate extracts at doses of 500, 50, and 500 microg per plate reduced benzo[a]pyrene mutagenicity by 95%, 82%, and 40%, respectively, in Salmonella typhimurium TA98 strain and by 91%, 66% and 63%, respectively, at the same doses with a TA97 assay system. Human lymphoblast cells K562 were pretreated with 50% inhibition concentration of each extracts and then treated by H(2)O(2), for the Comet assay. The Comet assay results showed that ethyl acetate and methanol extracts decreased the DNA damage caused by H(2)O(2) by, respectively, 34.8% and 31.3%. We envisaged also the study of the antioxidant effect of these extracts by the enzymatic xanthine/xanthine oxidase assay. Results indicated that methanol and ethyl acetate extracts were potent inhibitors of xanthine oxidase and superoxide anion scavengers. We conclude that these integrated approaches to antigenotoxicity and antioxidant assessment may be useful to help compare the beneficial effects associated with using A salicina as medicinal and dietary plant.
水提取物、甲醇提取物和乙酸乙酯提取物。通过使用艾姆斯试验评估间接作用诱变剂苯并[a]芘的致突变性抑制作用以及使用“彗星试验”评估直接作用诱变剂过氧化氢的遗传毒性，对这些提取物的抗遗传毒性特性进行了研究。在鼠伤寒沙门氏菌TA98菌株中，每平板500、50和500微克剂量的水提取物、甲醇提取物和乙酸乙酯提取物分别使苯并[a]芘的致突变性降低了95%、82%和40%；在TA97检测系统中，相同剂量下分别降低了91%、66%和63%。人淋巴母细胞K562用每种提取物的50%抑制浓度进行预处理，然后用H₂O₂处理，用于彗星试验。彗星试验结果表明，乙酸乙酯提取物和甲醇提取物分别使H₂O₂引起的DNA损伤降低了34.8%和31.3%。我们还设想通过酶促黄嘌呤/黄嘌呤氧化酶试验研究这些提取物的抗氧化作用。结果表明，甲醇提取物和乙酸乙酯提取物是黄嘌呤氧化酶的有效抑制剂和超氧阴离子清除剂。我们得出结论，这些综合的抗遗传毒性和抗氧化评估方法可能有助于比较将柳叶相思树用作药用和食用植物的相关有益效果。
