Qi Xiang-Yang, Chen Wei-Jun, Zhang Li-Qin, Xie Bi-Jun
College of Biological Environmental Sciences, Zhejiang Wali College, Ningbo 315100, China.
Nutr Res. 2008 Apr;28(4):278-84. doi: 10.1016/j.nutres.2008.02.008.
This study evaluated the supplementation of a mogrosides extract (MG) from fruits of Siraitia grosvenori on reducing oxidative stress, hyperglycemia, and hyperlipidemia in alloxan-induced diabetic mice. The oxygen free radical scavenging activity of MG was also assessed in vitro. After induction of diabetes, a significant increase in the levels of serum glucose, total cholesterol (TC), triacylglycerol (TG), and hepatic malondialdehyde (MDA) as well as a reduction in the level of hepatic high-density lipoprotein cholesterol (HDL-C) associated with diminution of the corresponding antioxidant enzymes, such as glutathione peroxidase (GSH-Px) and superoxide dismutase, were observed in all diabetic mice. Treatment of diabetic mice with MG (100, 300, and 500 mg/kg ) for 4 weeks significantly decreased serum glucose, TC, TG, and hepatic MDA levels (P < .05), whereas it increased serum HDL-C level and reactivated the hepatic antioxidant enzymes (P < .05) in alloxan-induced diabetic mice (P < .05). The hypoglycemic, hypolipidemic, and antioxidative activities of MG (100 mg/kg treatment) were all higher compared with all other diabetic groups and were similar to that observed for XiaoKeWan-pill (894 mg/kg; Guangzhou Zhongyi Pharmaceutical Co., Ltd., Guangzhou, China), a Chinese traditional antidiabetic drug. Antioxidant capacity evaluated in vitro showed that MG and mogroside V, which was the main component of MG, possessed strong oxygen free radical scavenging activities. These results demonstrate that the extract may have capacity to inhibiting hyperglycemia induced by diabetes, and the data suggest that administration of the extract may be helpful in the prevention of diabetic complications associated with oxidative stress and hyperlipidemia. We conclude that the extract should be evaluated as a candidate for future studies on diabetes mellitus.
本研究评估了罗汉果果实中罗汉果甜苷提取物（MG）对降低四氧嘧啶诱导的糖尿病小鼠氧化应激、高血糖和高血脂的作用。还在体外评估了MG的氧自由基清除活性。糖尿病诱导后，所有糖尿病小鼠的血清葡萄糖、总胆固醇（TC）、三酰甘油（TG）和肝脏丙二醛（MDA）水平显著升高，同时肝脏高密度脂蛋白胆固醇（HDL-C）水平降低，相应的抗氧化酶如谷胱甘肽过氧化物酶（GSH-Px）和超氧化物歧化酶减少。用MG（100、300和500mg/kg）治疗糖尿病小鼠4周，可显著降低血清葡萄糖、TC、TG和肝脏MDA水平（P<.05），同时提高血清HDL-C水平并重新激活肝脏抗氧化酶（P<.05）（P<.05）。与所有其他糖尿病组相比，MG（100mg/kg治疗）的降血糖、降血脂和抗氧化活性均更高，且与中国传统抗糖尿病药物消渴丸（894mg/kg；广州中一药业有限公司，广州，中国）相似。体外评估的抗氧化能力表明，MG及其主要成分罗汉果甜苷V具有很强的氧自由基清除活性。这些结果表明，该提取物可能具有抑制糖尿病诱导的高血糖的能力，数据表明给予该提取物可能有助于预防与氧化应激和高血脂相关的糖尿病并发症。我们得出结论，该提取物应作为未来糖尿病研究的候选物进行评估。
