牛、羊和山羊乳乳铁蛋白的电泳行为、构象、免疫化学性质及凝集素反应性比较。

Comparison of bovine, sheep and goat milk lactoferrins in their electrophoretic behavior, conformation, immunochemical properties and lectin reactivity.

Shimazaki K, Kawano N, Yoo Y C

Bioindustrial Food Chemistry Department, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan.

Comp Biochem Physiol B. 1991;98(2-3):417-22. doi: 10.1016/0305-0491(91)90199-n.

Abstract

The biochemical properties of bovine, goat and sheep lactoferrin were compared. Molecular weights of the three lactoferrins were estimated to be 78,000 to 80,000 as determined by SDS-PAGE. By IEF, microheterogeneity was observed for all of them. 2. Partial antigenic identity was observed between bovine lactoferrin and goat or sheep lactoferrin by immunodiffusion method. 3. CD spectra at the u.v. region of the three lactoferrins suggested their similar secondary and tertiary structural profiles. 4. Reactivities with peroxidase-conjugated lectins showed that the carbohydrate compositions of the three ruminants' lactoferrin were the same but not identical with that of human lactoferrin.

摘要

比较了牛、山羊和绵羊乳铁蛋白的生化特性。通过SDS-PAGE测定，三种乳铁蛋白的分子量估计为78,000至80,000。通过IEF观察到它们都存在微异质性。2. 通过免疫扩散法观察到牛乳铁蛋白与山羊或绵羊乳铁蛋白之间存在部分抗原同一性。3. 三种乳铁蛋白在紫外区域的圆二色光谱表明它们具有相似的二级和三级结构特征。4. 与过氧化物酶偶联凝集素的反应表明，三种反刍动物乳铁蛋白的碳水化合物组成相同，但与人类乳铁蛋白的不同。