使用Rotofor和色谱聚焦法分析人乳和牛乳中的乳铁蛋白。

Analysis of human and bovine milk lactoferrins by Rotofor and chromatofocusing.

Shimazaki K, Kawaguchi A, Sato T, Ueda Y, Tomimura T, Shimamura S

Protein Chemistry Section, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan.

Int J Biochem. 1993 Nov;25(11):1653-8. doi: 10.1016/0020-711x(93)90524-i.

Abstract

Isoelectric points of human and bovine lactoferrins were evaluated by Rotofor and chromatofocusing analysis. 2. By Rotofor, the isoelectric value of human lactoferrin fraction was determined at 8.7 and that of bovine lactoferrin at 8.8. 3. By chromatofocusing analysis, human and bovine lactoferrins showed different elution patterns. Human lactoferrin was eluted at pH 6.8-8 and bovine lactoferrin eluted at pH 8.2-8.9.

摘要

通过Rotofor和色谱聚焦分析评估了人乳铁蛋白和牛乳铁蛋白的等电点。2. 通过Rotofor测定，人乳铁蛋白组分的等电值为8.7，牛乳铁蛋白的等电值为8.8。3. 通过色谱聚焦分析，人乳铁蛋白和牛乳铁蛋白呈现出不同的洗脱模式。人乳铁蛋白在pH 6.8 - 8时洗脱，牛乳铁蛋白在pH 8.2 - 8.9时洗脱。