问题8——一个包含德国DNA数据库系统的短扩增子多重检测法。
Q8--a short amplicon multiplex including the German DNA database systems.
作者信息
Müller Kathrin, Klein Rachel, Miltner Erich, Wiegand Peter
机构信息
Institute of Legal Medicine, University Hospital Ulm, Germany.
出版信息
Forensic Sci Int Genet. 2007 Jun;1(2):205-7. doi: 10.1016/j.fsigen.2007.02.005. Epub 2007 Mar 26.
We have developed a modified STR (short tandem repeat) concept including the eight German DNA database systems D3S1358, FGA, TH01, VWA, SE33, D8S1179, D18S51, D21S11 and the gender typing system Amelogenin (Q8). The Q8 is well suitable especially for weak and degraded stains. Compared to commercially established DNA database kits the amplicon lengths were significantly reduced. To optimise the typing success for weak stains the most sensitive fluorescent dyes FAM, JOE and TET were used. By comparing the Q8 kit with the MPX2 kit, a well-established commercial kit, a higher success rate was reached using our concept.
我们开发了一种改良的短串联重复序列（STR）概念，包括八个德国DNA数据库系统：D3S1358、FGA、TH01、VWA、SE33、D8S1179、D18S51、D21S11以及性别分型系统牙釉蛋白（Q8）。Q8特别适用于微弱和降解的污渍。与商业上已确立的DNA数据库试剂盒相比，扩增子长度显著缩短。为了优化微弱污渍的分型成功率，使用了最灵敏的荧光染料FAM、JOE和TET。通过将Q8试剂盒与一种成熟的商业试剂盒MPX2试剂盒进行比较，我们的概念获得了更高的成功率。
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