Schmid D, Bayer B, Anslinger K
Institute of Legal Medicine, Ludwig-Maximilians-University of Munich, Nussbaumstr 26, 80335 Munich, Germany.
Forensic Sci Int Genet. 2008 Dec;3(1):22-6. doi: 10.1016/j.fsigen.2008.07.003. Epub 2008 Aug 8.
STR investigations of telogen hair are invariably difficult due to the small amounts of nuclear DNA and its degradation products. However, in recent years there has been a considerable improvement. This study examined the suitability of a new STR kit with shortened amplicons for the investigation of hair in routine casework. This kit allows the simultaneous amplification of the eight STR-loci D3S1358, VWA, FGA, TH01, SE33, D8S1179, D18S51, and D21S11, and the sex-determining amelogenin system. It was tested against the genRES MPX-SP1 and genRES MPX-SP2 kits. The sensitivity of the new genRES MPX-2SP kit was demonstrated to be inferior to that of the genRES MPX-SP1, but almost equal to that of the genRES MPX-SP2 kit.
由于休止期毛发中的核DNA及其降解产物含量很少，对其进行短串联重复序列（STR）分析一直都很困难。然而，近年来情况有了相当大的改善。本研究检验了一种具有更短扩增子的新型STR试剂盒在常规案件毛发检验中的适用性。该试剂盒能够同时扩增8个STR基因座，即D3S1358、VWA、FGA、TH01、SE33、D8S1179、D18S51和D21S11，以及用于性别鉴定的牙釉蛋白系统。该试剂盒与genRES MPX - SP1和genRES MPX - SP2试剂盒进行了对比测试。结果表明，新型genRES MPX - 2SP试剂盒的灵敏度低于genRES MPX - SP1试剂盒，但几乎与genRES MPX - SP2试剂盒相当。
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