海啸遇难者致密骨附着组织中的“外来”DNA。

"Foreign" DNA in tissue adherent to compact bone from tsunami victims.

作者信息

Zehner Richard

机构信息

Zentrum der Rechtsmedizin der J.W. Goethe Universität, Kennedyallee 104, D-60596 Frankfurt, Germany.

出版信息

Forensic Sci Int Genet. 2007 Jun;1(2):218-22. doi: 10.1016/j.fsigen.2007.01.006. Epub 2007 Feb 27.

DOI:10.1016/j.fsigen.2007.01.006

PMID:19083762

Abstract

We present two cases of tsunami victim identification where the DNA profile from compact bone gave an authentic DNA profile, while the DNA profiles obtained from adhering soft tissue or bone marrow gave different, incorrect profiles. The source of the contaminating DNA is unclear; the possibility that this occurred via transfer of putrefaction liquid in stacked bodies, or dispersal of contaminating material during conservation with formalin is discussed. We recommend the exclusive use of carefully sampled compact bone without adhering tissue as source of DNA to avoid misidentification in similar cases.

摘要

我们报告了两例海啸遇难者身份鉴定的案例，其中致密骨的DNA图谱给出了真实的DNA图谱，而从附着的软组织或骨髓中获得的DNA图谱则给出了不同的、错误的图谱。污染DNA的来源尚不清楚；讨论了这种情况是通过堆叠尸体中的腐败液体转移，还是在福尔马林保存过程中污染物质扩散而发生的可能性。我们建议仅使用仔细采样的无附着组织的致密骨作为DNA来源，以避免在类似案例中出现错误鉴定。