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用于灾难受害者身份识别(DVI)中DNA分析的股骨、肋骨和牙齿样本采集:一种将污染风险降至最低的方法。

Femur, rib, and tooth sample collection for DNA analysis in disaster victim identification (DVI) : a method to minimize contamination risk.

作者信息

Westen Antoinette A, Gerretsen Reza R R, Maat George J R

机构信息

Barge's Anthropologica, Leiden University Medical Center, Leiden, The Netherlands.

出版信息

Forensic Sci Med Pathol. 2008;4(1):15-21. doi: 10.1007/s12024-007-0027-0. Epub 2007 Aug 16.

Abstract

Although much literature is available on DNA extraction from tissue samples to obtain the best possible genotyping results, to the best of our knowledge no written recommendations exist on how to excise or extract bone and tooth samples from a victim to facilitate this. Because the possibility of cross-contamination is high, especially when excising numerous samples under disaster conditions, it is important to minimize this risk and to keep samples in optimum condition. In this paper a standard operating procedure is proposed for collection of femur, rib, and tooth samples to aid victim identification both after mass disasters and in (single) forensic investigations.

摘要

虽然有大量关于从组织样本中提取DNA以获得尽可能最佳基因分型结果的文献,但据我们所知,尚无关于如何从受害者身上切除或提取骨骼和牙齿样本以促进此过程的书面建议。由于交叉污染的可能性很高,尤其是在灾难情况下切除大量样本时,将这种风险降至最低并使样本保持最佳状态非常重要。本文提出了一种用于采集股骨、肋骨和牙齿样本的标准操作程序,以协助在大规模灾难后以及(单个)法医调查中进行受害者身份识别。

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