使用DNA IQ系统从信封封口处提取人类DNA用于STR基因分型的回收优化。
Optimization of recovery of human DNA from envelope flaps using DNA IQ System for STR genotyping.
作者信息
Ng Lay-Keow, Ng Andy, Cholette François, Davis Chris
机构信息
Laboratory and Scientific Services Directorate, Research and Development Division, Canada Border Services Agency, 79 Bentley Avenue, Ottawa, Ontario K1A 0L8, Canada.
出版信息
Forensic Sci Int Genet. 2007 Dec;1(3-4):283-6. doi: 10.1016/j.fsigen.2007.05.004. Epub 2007 Jul 13.
An optimized protocol based on the DNA IQ System has been tested for the extraction of DNA from envelope flaps. DNA is extracted directly without the need for opening and swabbing the flaps. The method is repeatable with <10% R.S.D. (relative standard deviation). The results of a systematic study show that it is an equilibrium extraction, and a small sample volume as well as high lysis buffer content in sample contribute to high extraction efficiency. The extracted DNA requires no further purification steps following its extraction with the DNA IQ System. Complete but skewed 15-locus short tandem repeat (STR) profiles, which is typical of degraded of DNA, have been generated from the DNA extracted from 6 to 9 years old casework envelope samples.
一种基于DNA IQ系统的优化方案已针对从信封封口处提取DNA进行了测试。无需打开并擦拭封口处即可直接提取DNA。该方法的相对标准偏差（R.S.D.）小于10%，具有可重复性。一项系统性研究结果表明，这是一种平衡提取方法，少量的样本体积以及样本中高含量的裂解缓冲液有助于提高提取效率。用DNA IQ系统提取的DNA无需进一步纯化步骤。从6至9年的案件信封样本中提取的DNA已生成完整但有偏差的15个位点的短串联重复（STR）图谱，这是DNA降解的典型特征。
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