Parsons Thomas J, Huel Rene, Davoren Jon, Katzmarzyk Cheryl, Milos Ana, Selmanović Arijana, Smajlović Lejla, Coble Michael D, Rizvić Adnan
International Commission on Missing Persons, 45A Alipasina, 71000 Sarajevo, Bosnia and Herzegovina.
Forensic Sci Int Genet. 2007 Jun;1(2):175-9. doi: 10.1016/j.fsigen.2007.02.003. Epub 2007 Mar 13.
The International Commission on Missing Persons (ICMP) conducts high throughput STR profiling on degraded skeletal remains, primarily recovered from mass graves relating to conflicts from 1992 to 1999 in the former Yugoslavia. To date, over 11,000 individuals have been identified through comparison of bone profiles to a large database of profiles from family members of the missing. To increase success rates in STR recovery, three short amplicon STR multiplexes (a 7-plex, a 6-plex, and a 5-plex) have been devised and implemented. These target loci from large commercial multiplexes, with an average decrease in amplicon size of 144 bp. The ICMP "miniplexes" have proven to provide substantially greater recovery of DNA data from a certain subset of difficult samples. However, the circumstances under which miniplexes provide additional data are restricted, and their advantages do not outweigh those of large commercial multiplexes for a majority of cases. The miniplexes, however, also have a very powerful use in DNA testing to support large scale reassociation of commingled, partial skeletons recovered from secondary mass graves.
国际失踪人员委员会（ICMP）对降解的骨骼遗骸进行高通量STR分型，这些遗骸主要从前南斯拉夫1992年至1999年冲突相关的乱葬坑中挖掘出来。迄今为止，通过将骨骼图谱与失踪人员家庭成员的大型图谱数据库进行比对，已确认了11000多人的身份。为提高STR回收成功率，已设计并实施了三种短扩增子STR复合扩增体系（一个7重体系、一个6重体系和一个5重体系）。这些体系针对大型商业复合扩增体系中的目标基因座，扩增子大小平均减少了144 bp。事实证明，ICMP的“微型复合扩增体系”能从某些难处理样本的子集中大幅提高DNA数据的回收率。然而，微型复合扩增体系提供额外数据的情况有限，在大多数情况下，其优势并不超过大型商业复合扩增体系。不过，微型复合扩增体系在DNA检测中也有非常强大的用途，可支持对从二次乱葬坑中挖掘出的混合、部分骨骼进行大规模重新关联。
