Sewell Jonathan, Quinones Ignacio, Ames Carole, Multaney Bryan, Curtis Stuart, Seeboruth Haj, Moore Stephen, Daniel Barbara
Department of Forensic Science and Drug Monitoring, King's College London, London, UK.
Forensic Sci Int Genet. 2008 Sep;2(4):281-5. doi: 10.1016/j.fsigen.2008.03.006. Epub 2008 May 16.
This study investigated the various factors affecting DNA profiling from DNA recovered from fingerprints deposited on paper before and after fingerprint enhancement treatments. The DNeasy plant mini kit (QIAGEN) was found to improve DNA recovery from paper by over 150% compared with the QIAamp mini kit. A significant decrease in the amount of DNA recovered was observed following treatment with DFO and/or Ninhydrin. This decrease in yield did not have a comparably significant effect on the quality of the SGM Plus profiles. Furthermore, this study found that whilst certain paper types, such as newspaper, magazine and filter paper allowed for the good recovery of DNA, common office paper and white card, strongly interfered with the recovery of DNA resulting in poor quality profiles.
本研究调查了指纹增强处理前后，从纸张上提取的指纹DNA进行DNA图谱分析时的各种影响因素。结果发现，与QIAamp微量提取试剂盒相比，DNeasy植物微量提取试剂盒（QIAGEN）可使从纸张中回收的DNA提高超过150%。在用DFO和/或茚三酮处理后，观察到回收的DNA量显著减少。产量的这种下降对SGM Plus图谱的质量没有同等显著的影响。此外，本研究发现，虽然某些纸张类型，如报纸、杂志和滤纸能较好地回收DNA，但普通办公用纸和白卡会强烈干扰DNA的回收，导致图谱质量较差。
