法医微量DNA：综述
Forensic trace DNA: a review.
作者信息
van Oorschot Roland Ah, Ballantyne Kaye N, Mitchell R John
机构信息
Forensic Services Department, Victoria Police, 31 Forensic Drive, Macleod 3085, Victoria, Australia.
出版信息
Investig Genet. 2010 Dec 1;1(1):14. doi: 10.1186/2041-2223-1-14.
DNA analysis is frequently used to acquire information from biological material to aid enquiries associated with criminal offences, disaster victim identification and missing persons investigations. As the relevance and value of DNA profiling to forensic investigations has increased, so too has the desire to generate this information from smaller amounts of DNA. Trace DNA samples may be defined as any sample which falls below recommended thresholds at any stage of the analysis, from sample detection through to profile interpretation, and can not be defined by a precise picogram amount. Here we review aspects associated with the collection, DNA extraction, amplification, profiling and interpretation of trace DNA samples. Contamination and transfer issues are also briefly discussed within the context of trace DNA analysis. Whilst several methodological changes have facilitated profiling from trace samples in recent years it is also clear that many opportunities exist for further improvements.
DNA分析经常被用于从生物材料中获取信息，以协助与刑事犯罪、灾难受害者身份鉴定和失踪人员调查相关的询问。随着DNA分析在法医调查中的相关性和价值不断增加，人们也越来越希望从更少量的DNA中获取这些信息。微量DNA样本可以被定义为在分析的任何阶段（从样本检测到图谱解读）低于推荐阈值的任何样本，并且不能用精确的皮克量来定义。在这里，我们回顾了与微量DNA样本的采集、DNA提取、扩增、分析和解读相关的方面。在微量DNA分析的背景下，也简要讨论了污染和转移问题。虽然近年来一些方法上的改变促进了微量样本的分析，但显然仍有许多进一步改进的机会。
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