AmpFℓSTR® NGM SElect™ PCR 扩增试剂盒的开发验证：一种带有 SE33 基因座的新一代 STR 多重扩增试剂盒。
Developmental validation of the AmpFℓSTR® NGM SElect™ PCR Amplification Kit: A next-generation STR multiplex with the SE33 locus.
机构信息
Life Technologies, 850 Lincoln Centre Dr., Foster City, CA 94404, United States.
出版信息
Forensic Sci Int Genet. 2013 Jan;7(1):41-51. doi: 10.1016/j.fsigen.2012.05.012. Epub 2012 Jun 27.
The AmpFℓSTR(®) NGM SElect™ PCR Amplification Kit is a new 17-plex STR genotyping kit designed for use primarily in forensic casework analysis. The kit was designed to be a counterpart to the AmpFℓSTR(®) NGM™ Kit for laboratories wishing to add the SE33 locus to the new European Standard Set of STR loci. The NGM SElect Kit shares the same primer sets for 16 common loci with the NGM Kit (D10S1248, D3S1358, vWA, D16S539, D2S1338, amelogenin, D8S1179, D21S11, D18S51, D19S433, TH01, FGA, D22S1045, D2S441, D1S1656 and D12S391), with additional primers for the SE33 locus. Developmental validation studies followed the Scientific Working Group on DNA Analysis Methods (SWGDAM) guidelines for STR kit manufacturers and tested several critical areas of kit performance including a sensitivity series, DNA mixtures and inhibited samples. The studies demonstrated that the NGM SElect Kit provides equivalent overall performance to the NGM Kit, but with even greater discriminatory power due to the inclusion of the highly informative SE33 locus.
AmpFℓSTR(®) NGM SElect™ PCR 扩增试剂盒是一种新的 17 重 STR 基因分型试剂盒，主要设计用于法医案件分析。该试剂盒旨在成为 AmpFℓSTR(®) NGM™试剂盒的对应物，供希望将 SE33 基因座添加到新的欧洲标准 STR 基因座组中的实验室使用。NGM SElect 试剂盒与 NGM 试剂盒（D10S1248、D3S1358、vWA、D16S539、D2S1338、amelogenin、D8S1179、D21S11、D18S51、D19S433、TH01、FGA、D22S1045、D2S441、D1S1656 和 D12S391）共享 16 个常见基因座的相同引物组，另外还有用于 SE33 基因座的引物。开发验证研究遵循 DNA 分析方法科学工作组 (SWGDAM) 对 STR 试剂盒制造商的指南，并测试了试剂盒性能的几个关键领域，包括灵敏度系列、DNA 混合物和抑制样本。这些研究表明，NGM SElect 试剂盒提供与 NGM 试剂盒相当的整体性能，但由于包含高度信息丰富的 SE33 基因座，因此具有更强的鉴别能力。
Zotero 插件