载脂蛋白B启动子功能多态性与丙型肝炎病毒感染的关联

Association of functional polymorphism of ApoB promoter with hepatitis C virus infection.

作者信息

Zhu Chengliang, Zhang Rui, Liu Dandan, Mukhtar Muhammad Mahmood, Liu Weiyong, Peng Guiqing, Wang Ke, Hao Qian, Xu Yilin, Liu Fang, Zhu Ying, Wu Jianguo

机构信息

The State Key Laboratory of Virology, College of Life Sciences and Chinese-French Liver Disease Research Institute, Zhongnan Hospital, Wuhan University, Wuhan, PR China.

出版信息

Clin Chim Acta. 2009 Mar;401(1-2):124-7. doi: 10.1016/j.cca.2008.12.001. Epub 2008 Dec 6.

DOI:10.1016/j.cca.2008.12.001

PMID:19083997

Abstract

BACKGROUND

Hepatitis C virus (HCV) binds to apolipoprotein B (ApoB) and low-density lipoprotein (LDL) before entering into hepatocytes. ApoB promoter polymorphisms influence the levels of ApoB and LDL in blood. We investigated the correlations between ApoB promoter polymorphism and HCV infection.

METHODS

ApoB promoter polymorphism was analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in 243 HCV-infected patients and 346 healthy individuals. Serum lipids concentrations were measured in all subjects.

RESULTS

ApoB polymorphisms on -516 position in patients were statistically different from healthy individuals (p<0.0001). CC genotype was higher in patients (58.7%) than in healthy individuals (43.1%) (p<0.0001). TT genotype was lower in patients (6.2%) than in healthy group (16.1%) (p<0.0001). C allele frequency was higher (76.3%) in patients than in healthy individuals (63.4%) (p<0.0001). T allele was lower in patients (23.7%) than in controls (36.6%) (p<0.0001). CC genotype was higher in HCV RNA positive patients (63.5%) than in negative ones (45.9%) (p<0.0001). TT genotype was significantly higher in HCV RNA negative patients (19.2%) than in positive individuals (2.6%) (p<0.0001).

CONCLUSIONS

CC genotype of ApoB promoter at -516 position may increase susceptibility of HCV infection and TT genotype may be associated with viral clearance.

摘要

背景

丙型肝炎病毒（HCV）在进入肝细胞之前会与载脂蛋白B（ApoB）和低密度脂蛋白（LDL）结合。ApoB启动子多态性会影响血液中ApoB和LDL的水平。我们研究了ApoB启动子多态性与HCV感染之间的相关性。

方法

采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）分析243例HCV感染患者和346例健康个体的ApoB启动子多态性。测量所有受试者的血脂浓度。

结果

患者-516位点的ApoB多态性与健康个体在统计学上有差异（p<0.0001）。患者中CC基因型（58.7%）高于健康个体（43.1%）（p<0.0001）。患者中TT基因型（6.2%）低于健康组（16.1%）（p<0.0001）。患者中C等位基因频率（76.3%）高于健康个体（63.4%）（p<0.0001）。患者中T等位基因（23.7%）低于对照组（36.6%）（p<0.0001）。HCV RNA阳性患者中CC基因型（63.5%）高于阴性患者（45.9%）（p<0.0001）。HCV RNA阴性患者中TT基因型（19.2%）显著高于阳性个体（2.6%）（p<0.0001）。