Liu Bao-Qiong, Zhang Le, Yang Qi-Dong, Liu Yun-Hai, Hu Zhong-Yang, Xu Hong-Wei, DU Xiao-Ping, Xia Jian
Department of Neurology, Xiangya Hospital, Central South University, Changsha 410008, China.
Zhonghua Yi Xue Za Zhi. 2008 Dec 23;88(47):3332-6.
To explore the relationship between apolipoprotein B (apoB) gene G12669A polymorphism and cerebral infarction with family history, and to evaluate the effect of G12669A polymorphism on plasma lipid levels.
Peripheral blood samples were collected from 147 members of 15 cerebral infarction families, including 47 cerebral infarction patients with positive family history (CIFH-P), 43 first-degree relatives (CIFH-I), 28 second-degree relatives (CIFH-II), and 29 third-degree relatives (CIFH-III), 83 sporadic cerebral infarction (SCI) patients, and 100 healthy controls. Polymerase chain reaction- restriction fragment length polymorphism was used to detect the apoB gene G12669A polymorphism. Oxidase method was used to detect the levels of triglyceride (TG), total cholesterol (TC), high-density lipoprotein (HDL), and low-density lipoprotein (LDL). The serum levels of lipoprotein (a) [LP (a)], apoB-100, and apoAI were determined by immune method.
(1) The frequencies of A allele in the CIFH-P, CIFH-I, CIFH-II, CIFH-III, and SCI groups patients and control group were 0.106, 0.081, 0.036, 0.034, 0.090, and 0.045 respectively, that of the CIFH-P group being significantly higher than that of the control group (P < 0.05), and those of the CIFH-I, CIFH-II, CIFH-III, and SCI groups not being significantly different from that of the control group. (2) In both CIFH-P and SCI groups, the TC and LDL-C levels of the patients with G/A gene type were significantly higher than those of the G/G gene type, while the HDL-C level of the patients with G/A gene type was significantly lower than that of the G/G gene type (all P < 0.05).
A allele in G12669A polymorphism may be one of the genetic factors influencing the susceptibility to CI in the individuals with a positive family history, and it may play its role through its influence on the blood lipid levels.
探讨载脂蛋白B（apoB）基因G12669A多态性与有家族史脑梗死的关系，评估G12669A多态性对血脂水平的影响。
收集15个脑梗死家系的147名成员的外周血样本，包括47名有家族史的脑梗死患者（CIFH-P）、43名一级亲属（CIFH-I）、28名二级亲属（CIFH-II）和29名三级亲属（CIFH-III），83名单发性脑梗死（SCI）患者，以及100名健康对照者。采用聚合酶链反应-限制性片段长度多态性方法检测apoB基因G12669A多态性。采用氧化酶法检测甘油三酯（TG）、总胆固醇（TC）、高密度脂蛋白（HDL）和低密度脂蛋白（LDL）水平。采用免疫法测定血清脂蛋白（a）[LP（a）]、apoB-100和apoAI水平。
（1）CIFH-P组、CIFH-I组、CIFH-II组、CIFH-III组、SCI组患者及对照组A等位基因频率分别为0.106、0.081、0.036、0.034、0.090和0.045，CIFH-P组显著高于对照组（P<0.05），CIFH-I组、CIFH-II组、CIFH-III组和SCI组与对照组差异无统计学意义。（2）在CIFH-P组和SCI组中，G/A基因型患者的TC和LDL-C水平均显著高于G/G基因型患者，而G/A基因型患者的HDL-C水平显著低于G/G基因型患者（均P<0.05）。
G12669A多态性中的A等位基因可能是影响有家族史个体对脑梗死易感性的遗传因素之一，且可能通过影响血脂水平发挥作用。
