Williams N D, Russell A D
Pharmaceutical Microbiology Research Laboratory, Welsh School of Pharmacy, University of Wales College of Cardiff, UK.
J Appl Bacteriol. 1991 May;70(5):427-36. doi: 10.1111/j.1365-2672.1991.tb02960.x.
Sodium hypochlorite (NaOCl) and sodium dichloroisocyanurate (NaDCC) were more active against Bacillus subtilis 8236 spores in both viability and in germination and outgrowth studies than were polyvinylpyrrolidone-iodine (PVP-I) and Lugol's solution. Of the two chlorine compounds studied NaOCl proved to be the more active. The two iodine-containing compounds gave contrasting results with the Lugol's solution demonstrating increased antibacterial activity with increasing available iodine concentration. The antibacterial behaviour of PVP-I, however, reflected the more complex nature of aqueous iodine-surfactant mixtures. Initially, non-complexed iodine concentration (the active species) increased with increasing total available iodine concentration, resulting in increasing antibacterial activity. However, due to changes in the physical properties of the mixture, a maximum concentration of non-complexed iodine was reached so that a further increase in total available iodine resulted in a decrease in non-complexed iodine concentration and consequently a decrease in the antibacterial activity of the solution was observed. A greater inhibitory effect was observed in subsequent germination and outgrowth studies when spores were pre-treated with respective biocide than when untreated spores were added to germination media containing biocide at t = 0. This may reflect a combination of different contact times plus the neutralizing effect of the germination media on such halogen compounds.
在活力、萌发及生长研究中，次氯酸钠（NaOCl）和二氯异氰尿酸钠（NaDCC）对枯草芽孢杆菌8236孢子的活性比聚乙烯吡咯烷酮碘（PVP-I）和卢戈氏溶液更强。在所研究的两种含氯化合物中，NaOCl被证明活性更强。两种含碘化合物给出了对比结果，卢戈氏溶液显示随着有效碘浓度增加，抗菌活性增强。然而，PVP-I的抗菌行为反映了碘-表面活性剂水性混合物更复杂的性质。最初，非络合碘浓度（活性成分）随着总有效碘浓度增加而升高，导致抗菌活性增强。然而，由于混合物物理性质的变化，非络合碘达到最大浓度，因此总有效碘进一步增加导致非络合碘浓度降低，结果观察到溶液的抗菌活性下降。在随后的萌发和生长研究中，当孢子用相应的杀菌剂预处理时，比在t = 0时将未处理的孢子添加到含有杀菌剂的萌发培养基中观察到更大的抑制作用。这可能反映了不同接触时间的综合作用以及萌发培养基对此类卤素化合物的中和作用。
