Wang Shengfu, Xie Fen, Liu Guodong
College of Chemistry and Chemical Engineering, Hubei University, Wuhan, PR China.
Talanta. 2009 Feb 15;77(4):1343-50. doi: 10.1016/j.talanta.2008.09.019. Epub 2008 Sep 19.
Direct electrochemistry and electrocatalysis of heme proteins including hemoglobin (Hb), myoglobin (Mb) and horseradish peroxidase (HRP) were studied with the protein incorporated single walled carbon nanotubes (SWCNTs)-cetylramethylammonium bromide (CTAB) nanocomposite film modified glassy carbon electrodes (GCEs). The incorporated heme proteins were characterized with Fourier transform infrared spectroscopy (FTIR), ultraviolet visible (UV) spectroscopy, atomic force microscopy (AFM) and electrochemistry, indicating the heme proteins in SWCNTs-CTAB nanocomposite films keep their secondary structure similar to their native states. The direct electron transfer between the heme proteins in SWCNTs-CTAB films and GCE was investigated. The electrochemical parameters such as formal potentials and apparent heterogeneous electrontransfer rate constants (k(s)) were estimated by square wave voltammetry with nonlinear regression analysis. The heme protein-SWCNT-CTAB electrodes show excellent electrocatalytic activities for the reduction of H(2)O(2) and NO(2)(-), which have been utilized to determine the concentrations of H(2)O(2) and NO(2)(-).
利用蛋白质掺杂的单壁碳纳米管（SWCNTs）-十六烷基三甲基溴化铵（CTAB）纳米复合膜修饰玻碳电极（GCEs），研究了包括血红蛋白（Hb）、肌红蛋白（Mb）和辣根过氧化物酶（HRP）在内的血红素蛋白的直接电化学和电催化性能。采用傅里叶变换红外光谱（FTIR）、紫外可见（UV）光谱、原子力显微镜（AFM）和电化学方法对掺杂的血红素蛋白进行了表征，结果表明SWCNTs-CTAB纳米复合膜中的血红素蛋白保持了与其天然状态相似的二级结构。研究了SWCNTs-CTAB膜中血红素蛋白与GCE之间的直接电子转移。通过方波伏安法结合非线性回归分析估算了形式电位和表观异相电子转移速率常数（k(s)）等电化学参数。血红素蛋白-SWCNT-CTAB电极对H(2)O(2)和NO(2)(-)的还原表现出优异的电催化活性，已用于测定H(2)O(2)和NO(2)(-)的浓度。
