Niu Yanyan, Xie Hui, Luo Guiling, Weng Wenju, Ruan Chengxiang, Li Guangjiu, Sun Wei
Key Laboratory of Tropical Medicinal Plant Chemistry of Ministry of Education, College of Chemistry and Chemical Engineering, Hainan Normal University Haikou 571158 P. R. China
Key Laboratory of Optic-Electric Sensing and Analytical Chemistry for Life Science of Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology Qingdao 266042 P. R. China.
RSC Adv. 2019 Feb 5;9(8):4480-4487. doi: 10.1039/c8ra07910b. eCollection 2019 Jan 30.
A new biosensing strategy based on a TiO-doped carbon nanofiber (CNF) composite modified electrode was developed. TiO@CNF was prepared by electrospinning with further carbonization, before being characterized by various methods and used for electrode modification on the surface of carbon ionic liquid electrode (CILE). Myoglobin (Mb) was further immobilized on the modified electrode surface. The results of ultraviolet-visible (UV-vis) and Fourier transform infrared (FT-IR) spectroscopy showed that Mb maintained its native structure without denaturation in the composite film. Direct electron transfer and the electrocatalytic properties of Mb on the electrode surface were further investigated. A pair of quasi-reversible redox peaks appeared on the cyclic voltammogram, indicating that direct electrochemistry of Mb was realized in the nanocomposite film. This could be attributed to the specific properties of TiO@CNF nanocomposite, including a large surface-to-volume ratio, good biocompatibility and high conductivity. Nafion/Mb/TiO@CNF/CILE exhibited an excellent electrochemical catalytic ability in the reduction of trichloroacetic acid, NaNO and HO. All results demonstrated potential applications of TiO@CNF in third-generation electrochemical biosensors.
基于二氧化钛掺杂碳纳米纤维（CNF）复合修饰电极开发了一种新的生物传感策略。通过静电纺丝并进一步碳化制备了TiO@CNF，然后用各种方法对其进行表征，并用于碳离子液体电极（CILE）表面的电极修饰。将肌红蛋白（Mb）进一步固定在修饰电极表面。紫外可见（UV-vis）光谱和傅里叶变换红外（FT-IR）光谱结果表明，Mb在复合膜中保持其天然结构而未变性。进一步研究了Mb在电极表面的直接电子转移和电催化性能。循环伏安图上出现了一对准可逆的氧化还原峰，表明在纳米复合膜中实现了Mb的直接电化学。这可归因于TiO@CNF纳米复合材料的特殊性质，包括大的比表面积、良好的生物相容性和高导电性。Nafion/Mb/TiO@CNF/CILE在三氯乙酸、NaNO和H₂O₂的还原反应中表现出优异的电化学催化能力。所有结果证明了TiO@CNF在第三代电化学生物传感器中的潜在应用。
