Wang Jianwen, Wang Liping, Di Junwei, Tu Yifeng
Department of Chemistry, Suzhou University, Suzhou, Jiangsu, PR China.
Talanta. 2009 Feb 15;77(4):1454-9. doi: 10.1016/j.talanta.2008.09.034. Epub 2008 Sep 30.
A novel disposable third-generation hydrogen peroxide (H(2)O(2)) biosensor based on horseradish peroxidase (HRP) immobilized on the gold nanoparticles (AuNPs) electrodeposited indium tin oxide (ITO) electrode is investigated. The AuNPs deposited on ITO electrode were characterized by UV-vis, SEM, and electrochemical methods. The AuNPs attached on the ITO electrode surface with quasi-spherical shape and the average size of diameters was about 25 nm with a quite symmetric distribution. The direct electron chemistry of HRP was realized, and the biosensor exhibited excellent performances for the reduction of H(2)O(2). The amperometric response to H(2)O(2) shows a linear relation in the range from 8.0 micromol L(-1) to 3.0 mmol L(-1) and a detection limit of 2 micromol L(-1) (S/N=3). The K(M)(app) value of HRP immobilized on the electrode surface was found to be 0.4 mmol L(-1). The biosensor indicates excellent reproducibility, high selectivity and long-term stability.
研究了一种基于固定在电沉积铟锡氧化物（ITO）电极上的金纳米颗粒（AuNPs）上的辣根过氧化物酶（HRP）的新型一次性第三代过氧化氢（H₂O₂）生物传感器。通过紫外可见光谱、扫描电子显微镜和电化学方法对沉积在ITO电极上的AuNPs进行了表征。附着在ITO电极表面的AuNPs呈准球形，平均直径约为25nm，分布相当对称。实现了HRP的直接电子化学，该生物传感器对H₂O₂的还原表现出优异的性能。对H₂O₂的安培响应在8.0 μmol L⁻¹至3.0 mmol L⁻¹范围内呈线性关系，检测限为2 μmol L⁻¹（S/N = 3）。发现固定在电极表面的HRP的Kₘₐₚ值为0.4 mmol L⁻¹。该生物传感器具有优异的重现性、高选择性和长期稳定性。
