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用于快速直接电化学检测接种了……的番茄叶片中过氧化氢的纸质分析装置

Paper-Based Analytical Devices for the Rapid and Direct Electrochemical Detection of Hydrogen Peroxide in Tomato Leaves Inoculated with .

作者信息

Sun Lijun, Pan Yu, Wu Jin, Zhao Danyang, Hui Meiqi, Zhu Suqin, Zhu Xinyu, Li Dayong, Song Fengming, Zhang Cankui

机构信息

Department of Biological Sciences, School of Life Sciences, Nantong University, 9 Seyuan Rd, Nantong 226019, Jiangsu, China.

National Key Laboratory for Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310029, Zhejiang, China.

出版信息

Sensors (Basel). 2020 Sep 26;20(19):5512. doi: 10.3390/s20195512.

DOI:10.3390/s20195512
PMID:32993080
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7582799/
Abstract

Hydrogen peroxide (HO) is an important signaling molecule and plays key roles in multiple plant physiological processes. The rapid and direct monitoring of HO could improve our understanding of its regulatory mechanisms in plants. In this study, we developed a paper-based analytical device consisting of a disposable nano-gold modified indium tin oxide working electrode to provide a platform for the rapid and direct detection of HO. The total analytical time was dramatically shortened to be approximate 3 min due to the avoidance of the time-consuming and complex treatment of plant samples. In addition, the amount of plant samples required was less than 3 mg in our approach. We used this system to monitor the concentrations of HO in tomato leaves infected by within 24 h. Our results showed that the concentration of HO in tomato leaves was increased in the initial phase, peaked at 1.5 μmol gFW at 6 h, and then decreased. The production trend of HO in tomato leaves inoculated with detected with our approach is similar to the 3,3-diaminobenzidine staining method. Taken together, our study offers a rapid and direct approach for the detection of HO, which will not only pave the way for the further investigation of the regulation mechanisms of HO in plants, but also promote the development of precision agriculture technology.

摘要

过氧化氢（HO）是一种重要的信号分子，在多种植物生理过程中发挥关键作用。对HO的快速直接监测有助于我们更好地理解其在植物中的调控机制。在本研究中，我们开发了一种基于纸的分析装置，其包含一个一次性纳米金修饰的氧化铟锡工作电极，为HO的快速直接检测提供了一个平台。由于避免了对植物样品进行耗时且复杂的处理，总分析时间大幅缩短至约3分钟。此外，我们的方法所需植物样品量少于3毫克。我们使用该系统在24小时内监测了感染[具体病原体未提及]的番茄叶片中HO的浓度。结果表明，番茄叶片中HO的浓度在初始阶段升高，在6小时时达到峰值1.5微摩尔每克鲜重，然后下降。用我们的方法检测接种[具体病原体未提及]的番茄叶片中HO的产生趋势与3,3 - 二氨基联苯胺染色法相似。综上所述，我们的研究提供了一种快速直接检测HO的方法，这不仅将为进一步研究HO在植物中的调控机制铺平道路，还将推动精准农业技术的发展。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d19d/7582799/5787156ab4ff/sensors-20-05512-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d19d/7582799/a42e2b994f8c/sensors-20-05512-sch001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d19d/7582799/9e81bb61a5e8/sensors-20-05512-sch002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d19d/7582799/3004e3e19eae/sensors-20-05512-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d19d/7582799/0b6ef58635c0/sensors-20-05512-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d19d/7582799/0ef2feb1a042/sensors-20-05512-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d19d/7582799/9605458079f5/sensors-20-05512-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d19d/7582799/5c7d408e1727/sensors-20-05512-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d19d/7582799/5787156ab4ff/sensors-20-05512-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d19d/7582799/a42e2b994f8c/sensors-20-05512-sch001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d19d/7582799/9e81bb61a5e8/sensors-20-05512-sch002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d19d/7582799/3004e3e19eae/sensors-20-05512-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d19d/7582799/0b6ef58635c0/sensors-20-05512-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d19d/7582799/0ef2feb1a042/sensors-20-05512-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d19d/7582799/9605458079f5/sensors-20-05512-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d19d/7582799/5c7d408e1727/sensors-20-05512-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d19d/7582799/5787156ab4ff/sensors-20-05512-g006.jpg

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