He Huan, Zhang Cheng-Gui, Xia Jin-Lan, Peng An-An, Yang Yi, Jiang Hong-Chen, Zheng Lei, Ma Chen-Yan, Zhao Yi-Dong, Nie Zhen-Yuan, Qiu Guan-Zhou
Key Laboratory of Biometallurgy of Ministry of Education of China, School of Minerals Processing & Bioengineering, Central South University, Changsha, Hunan 410083, China.
Curr Microbiol. 2009 Apr;58(4):300-7. doi: 10.1007/s00284-008-9330-6. Epub 2008 Dec 16.
The speciation transformation of elemental sulfur mediated by the leaching bacterium Acidithiobacillus ferrooxidans was investigated using an integrated approach including scanning electron microscopy, transmission electron microscopy, Fourier transform infrared spectroscopy, energy dispersive X-ray spectroscopy, and X-ray absorption near edge spectroscopy (XANES). Our results showed that when grown on elemental sulfur powder, At. ferrooxidans ATCC23270 cells were first attached to sulfur particles and modified the surface sulfur with some amphiphilic compounds. In addition, part of the elemental sulfur powder might be converted to polysulfides. Furthermore, sulfur globules were accumulated inside the cells. XANES spectra of these cells suggested that these globules consisted of elemental sulfur bound to thiol groups of protein.
利用包括扫描电子显微镜、透射电子显微镜、傅里叶变换红外光谱、能量色散X射线光谱和X射线吸收近边光谱（XANES）在内的综合方法，研究了浸矿细菌氧化亚铁硫杆菌介导的元素硫的形态转化。我们的结果表明，当在元素硫粉末上生长时，氧化亚铁硫杆菌ATCC23270细胞首先附着在硫颗粒上，并用一些两亲性化合物修饰表面硫。此外，部分元素硫粉末可能转化为多硫化物。此外，硫球在细胞内积累。这些细胞的XANES光谱表明，这些球由与蛋白质巯基结合的元素硫组成。
