Prange A, Arzberger I, Engemann C, Modrow H, Schumann O, Trüper H G, Steudel R, Dahl C, Hormes J
Institut für Mikrobiologie & Biotechnologie, Rheinische Friedrich-Wilhelms-Universität Bonn, Meckenheimer Allee 168, D-53115, Bonn, Germany.
Biochim Biophys Acta. 1999 Aug 5;1428(2-3):446-54. doi: 10.1016/s0304-4165(99)00095-1.
During the oxidation of sulfide and thiosulfate purple and green sulfur bacteria accumulate globules of 'elemental' sulfur. Although essential for a thorough understanding of sulfur metabolism in these organisms, the exact chemical nature of the stored sulfur is still unclear. We applied sulfur K-edge X-ray absorption near edge spectroscopy (XANES) to probe the forms of sulfur in intact cells. Comparing XANES spectra of Allochromatium vinosum, Thiocapsa roseopersicina, Marichromatium purpuratum, Halorhodospira halophila and Chlorobium vibrioforme grown photolithoautotrophically on sulfide with reference probes (fingerprint method), we found sulfur chains with the structure R-S(n)-R. Evidence for the presence of sulfur rings, polythionates and anionic polysulfides in the sulfur globules of these bacteria was not obtained.
在硫化物和硫代硫酸盐氧化过程中，紫色和绿色硫细菌会积累“元素”硫的颗粒。尽管对于全面了解这些生物体中的硫代谢至关重要，但所储存硫的确切化学性质仍不清楚。我们应用硫K边X射线吸收近边光谱法（XANES）来探测完整细胞中硫的形态。通过将在硫化物上光自养生长的嗜酒色杆菌、玫瑰色硫囊菌、紫色嗜盐菌、嗜盐嗜盐红螺菌和绿弯菌的XANES光谱与参考探针（指纹法）进行比较，我们发现了具有R-S(n)-R结构的硫链。在这些细菌的硫颗粒中未获得存在硫环、连多硫酸盐和阴离子多硫化物的证据。
