ICFO-Institut de Ciències Fotòniques, Mediterranean Technology Park, 08860, Castelldefels, Barcelona, Spain.
Cytotechnology. 2008 Oct;58(2):103-11. doi: 10.1007/s10616-008-9178-4. Epub 2008 Dec 16.
A 3 mW focused femtosecond laser spot at a distance (>15 mum) has shown to attract the fillopodia from growth cones of primary neuronal cell cultures (mice E15). The phenomenological behavior of fillopodia is studied under short durations (~40 min) and different laser light conditions. The analysis of the fillopodia movement showed that they become significantly attracted towards the focused femtosecond laser light. In contrast, the use of continuous wave under the same conditions did not generate the same effect, the results of which were indistinguishable from when there was no laser light present (control condition). These results suggest the possible existence of an optically-induced signaling mechanism in growth cones.
一个 3mW 的聚焦飞秒激光光斑在距离（>15 微米）处被证明可以吸引原代神经元细胞培养物（E15 期小鼠）的生长锥的丝状伪足。在短时间（~40 分钟）和不同激光光条件下研究了丝状伪足的现象性行为。对丝状伪足运动的分析表明，它们明显地被聚焦的飞秒激光吸引。相比之下，在相同条件下使用连续波不会产生相同的效果，其结果与没有激光光存在时（对照条件）无法区分。这些结果表明，在生长锥中可能存在光诱导信号机制。
