Department of Chemical and Materials Engineering, National Central University, No. 300, Jhongda Rd., Jhongli, Taoyuan, 32001, Taiwan,
Cytotechnology. 2007 Jul;54(3):181-8. doi: 10.1007/s10616-007-9087-y. Epub 2007 Aug 15.
The neurite outgrowth of PC12 cells on collagen-coated glass plates under light emitting diode (LED) irradiation at several wavelengths (i.e., 455, 470, 525, 600, 630, 880 and 945 nm) was investigated. No neurite outgrowth was observed during cultivation under irradiation from the lamp of an inverted light microscope through filters (yielding mixed light at ca. 525 nm and more than 800 nm), whereas neurite outgrowth was observed during cultivation in the dark. When these cells were irradiated with monochromatic LED light, neurite outgrowth was slightly, but not completely, suppressed at 455, 525, 600, 630, 880 and 945 nm, as was observed in the case of mixed light. Long connected neuronal outgrowths (e.g., 3 mm length) were observed with LED light at 470 nm and 1.8 mW/cm(2) intensity. No such outgrowths were observed at other LED light wavelengths (i.e., 455, 525, 600, 630, 880 and 945 nm). Irradiation at 470 nm may have caused specific responses to transductional signals in these cells that led to the connection of neuronal outgrowths between cells. Not only suppressed neurite outgrowth but also long connected neurite outgrowths were observed when PC12 cells were cultured under several different wavelengths of light.
研究了在发光二极管（LED）照射下，几种波长（即 455、470、525、600、630、880 和 945nm）的胶原涂覆玻璃平板上 PC12 细胞的突起生长。在通过滤光片（产生约 525nm 和 800nm 以上的混合光）的倒置显微镜灯照射下培养时，没有观察到突起生长，而在黑暗中培养时则观察到突起生长。当这些细胞用单色 LED 光照射时，在 455、525、600、630、880 和 945nm 处观察到的混合光下，突起生长略有但不完全受到抑制。在 470nm 和 1.8mW/cm2 强度的 LED 光下观察到长的神经元突起生长（例如 3mm 长度）。在其他 LED 光波长（即 455、525、600、630、880 和 945nm）处未观察到这种生长。470nm 的照射可能导致了这些细胞中转导信号的特定反应，从而导致了细胞间神经元突起的连接。在几种不同波长的光下培养 PC12 细胞时，不仅观察到突起生长受到抑制，而且还观察到长的神经元突起生长。
