Vasey D B, Lillico S G, Sang H M, King T J, Whitelaw C B A
Division of Developmental Biology, The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Roslin, Midlothian, EH25 9PS, Scotland, UK.
Transgenic Res. 2009 Apr;18(2):309-14. doi: 10.1007/s11248-008-9235-y. Epub 2008 Dec 16.
The CMV enhancer-promoter sequence is often used as a transcriptional regulatory element in vector systems. We have used this control element to drive expression of GFP in a lentivirus vector transgene in pigs and chickens. Promoted as a 'universal' enhancer/promoter element capable of transcriptional activity in a number of cells in vitro, CMV-GFP transgene expression in vivo is preferentially observed in exocrine cells. This expression profile validates the use of this transcriptional control sequence to target expression to exocrine cells in gene transfer strategies.
巨细胞病毒增强子-启动子序列经常在载体系统中用作转录调控元件。我们已使用这个调控元件在猪和鸡的慢病毒载体转基因中驱动绿色荧光蛋白（GFP）的表达。作为一种能够在多种体外培养细胞中具有转录活性的“通用”增强子/启动子元件进行推广，体内的巨细胞病毒-绿色荧光蛋白转基因表达优先在外分泌细胞中观察到。这种表达谱验证了在基因转移策略中使用这个转录控制序列将表达靶向到外分泌细胞的可行性。
