Calvez S, Prévost H, Drider D
Laboratoire de Microbiologie, Ecole Nationale d'Ingénieurs des Techniques des Industries Agricoles et Alimentaires, 44322, Nantes Cedex 3, France.
Folia Microbiol (Praha). 2008;53(5):417-22. doi: 10.1007/s12223-008-0063-5. Epub 2008 Dec 16.
It was shown recently (Calvez et al. 2007) that glpQ and pde genes are involved in intermediate resistance of Enterococcus faecalis JH2-2 to DvnV41, a class IIa bacteriocin produced by Carnobacterium divergens V41. The listerial orthologs of lpQ and pde genes might be lmo0052 and lmo1292 genes, respectively. Here, the role of lmo0052 and lmo1292 genes in resistance of Listeria monocytogenes EGDe to DvnV41 and MesY105 was investigated. L. monocytogenes EGDe was inactivated in lmo0052 and/or lmo1292 by homologous recombination. Listerial mutant strain EGDSC02 (inactivated in the putative glpQ gene), was slightly resistant to DvnV41. The listerial mutant strain EGDSC01 (inactivated in the putative pde gene) remained, as the wild-type strain, sensitive to DvnV41, but was affected in growth parameters.
最近有研究表明（卡尔维兹等人，2007年），glpQ和pde基因参与粪肠球菌JH2-2对DvnV41的中度抗性，DvnV41是一种由分歧肉杆菌V41产生的IIa类细菌素。lpQ和pde基因在李斯特菌中的直系同源基因可能分别是lmo0052和lmo1292基因。在此，研究了lmo0052和lmo1292基因在单核细胞增生李斯特菌EGDe对DvnV41和MesY105抗性中的作用。通过同源重组使单核细胞增生李斯特菌EGDe的lmo0052和/或lmo1292基因失活。李斯特菌突变株EGDSC02（假定的glpQ基因失活）对DvnV41有轻微抗性。李斯特菌突变株EGDSC01（假定的pde基因失活）与野生型菌株一样，对DvnV41敏感，但生长参数受到影响。
