Higuchi Masayuki, Kakimoto Tatsuo, Mizuno Takeshi
Laboratory of Plant Molecular Genetics, Nara Institute of Science and Technology, Takayama, Ikoma, Japan.
Methods Mol Biol. 2009;495:101-9. doi: 10.1007/978-1-59745-477-3_9.
The cytokinin class of plant hormones is perceived by transmembrane His-kinases (His-kinases) of the two-component system, otherwise known as the His-Asp phosphorelay system. When cytokinin receptors perceive cytokinins, they are autophosphorylated at a conserved His residue. The phosphoryl group is then transferred to downstream components of the His-Asp phosphorelay system. When the gene for a cytokinin receptor is introduced into yeast or Escherichia coli, the corresponding receptor feeds the phosphoryl group to the phosphorelay system of the host, in a cytokinin-dependent manner. Therefore, these microorganisms can be used as convenient cytokinin sensors, and can also be used to understand the properties of cytokinin-receptors. Furthermore, they may be used to screen for cytokinin agonists and antagonists, which would potentially be useful to regulate the growth of crops.
植物激素细胞分裂素类是由双组分系统的跨膜组氨酸激酶（组氨酸激酶）感知的，双组分系统也被称为组氨酸-天冬氨酸磷酸传递系统。当细胞分裂素受体感知到细胞分裂素时，它们会在一个保守的组氨酸残基上进行自磷酸化。然后磷酸基团被转移到组氨酸-天冬氨酸磷酸传递系统的下游组分。当将细胞分裂素受体基因导入酵母或大肠杆菌时，相应的受体以细胞分裂素依赖的方式将磷酸基团提供给宿主的磷酸传递系统。因此，这些微生物可以用作方便的细胞分裂素传感器，也可用于了解细胞分裂素受体的特性。此外，它们可用于筛选细胞分裂素激动剂和拮抗剂，这可能对调节作物生长有用。
