Maier U, Kirchheimer J C, Hienert G, Christ G, Binder B R
Department of Urology, University of Vienna, Austria.
J Urol. 1991 Sep;146(3):906-8. doi: 10.1016/s0022-5347(17)37958-2.
Urokinase-type (u-PA) and tissue-type plasminogen activator antigen (t-PA) as well as plasminogen activator-inhibitor activity were determined in seminal plasma and lysates of the respective spermatozoas in 67 ejaculate of males in infertile marriage without genito urinary pathology. U-PA was determined by a competition RIA, t-PA by an ELISA and PAI by a spectrophotometric assay. 15 patients showed normozoospermia, 11 azoospermia and 41 oligoasthenoteratozoospermia (OAT-syndrome). In lysates of spermatozoas, significantly higher levels of both plasminogenactivators and PAI were found in patients with OAT syndrome as compared to those exhibiting normozoospermia. Whereas PAI was absent in the seminal plasma of normozoospermic ejaculate, patients with azoospermia (180 +/- 13 mU/ml.) and OAT-syndrome (60 +/- 5 mU/ml.) showed high PAI levels. The similarly high values of t-PA (190.8-227.8 ng./ml.) and u-PA (19.4-32 ng./ml.) in the same compartment confirm their predominantly prostatic origin and seem to have no influence on the quality of the ejaculate.
在67例无泌尿生殖系统病变的不育男性的精液及相应精子裂解物中，测定了尿激酶型纤溶酶原激活剂（u-PA）、组织型纤溶酶原激活剂抗原（t-PA）以及纤溶酶原激活剂抑制剂活性。u-PA采用竞争放射免疫分析法测定，t-PA采用酶联免疫吸附测定法测定，PAI采用分光光度法测定。15例患者表现为正常精子症，11例无精子症，41例为少弱畸精子症（OAT综合征）。与正常精子症患者相比，OAT综合征患者的精子裂解物中纤溶酶原激活剂和PAI水平显著更高。正常精子症精液的精浆中不存在PAI，而无精子症患者（180±13 mU/ml）和OAT综合征患者（60±5 mU/ml）的PAI水平较高。同一组分中t-PA（190.8 - 227.8 ng/ml）和u-PA（19.4 - 32 ng/ml）的相似高值证实了它们主要来源于前列腺，且似乎对精液质量没有影响。
