• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利:CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法,用中文像聊天一样搜索,搜遍4000万医学文献。AI智能推荐,让科研检索更轻松。

立即免费搜索

文件翻译

保留排版,准确专业,支持PDF/Word/PPT等文件格式,支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述,25分钟生成高质量综述,智能提取关键信息,辅助科研写作。

立即免费体验

胚胎器官培养

Embryonic organ culture.

作者信息

Sakai Takayoshi, Onodera Tomohiro

机构信息

Department of Oral-Facial Disorders, Division of Functional Oral Neuroscience, Osaka University Graduate School of Dentistry, Osaka, Japan.

出版信息

Curr Protoc Cell Biol. 2008 Dec;Chapter 19:Unit 19.8. doi: 10.1002/0471143030.cb1908s41.

DOI:10.1002/0471143030.cb1908s41
PMID:19085985
Abstract

This unit provides detailed protocols for dissecting embryonic organs and performing organ culture to study questions in developmental biology. Procedures are described here for dissecting organs such as kidney, lung, and salivary gland. The unit also contains commentary including troubleshooting for embryonic organ culture.

摘要

本单元提供了用于解剖胚胎器官和进行器官培养以研究发育生物学问题的详细方案。这里描述了诸如肾脏、肺和唾液腺等器官的解剖程序。本单元还包含相关评论,包括胚胎器官培养的故障排除。

相似文献

1
Embryonic organ culture.胚胎器官培养
Curr Protoc Cell Biol. 2008 Dec;Chapter 19:Unit 19.8. doi: 10.1002/0471143030.cb1908s41.
2
Growth and morphogenesis of embryonic mouse organs on non-coated and extracellular matrix-coated Biopore membrane.
Dev Growth Differ. 1993;35(6):683-90. doi: 10.1111/j.1440-169x.1993.00683.x.
3
Dissecting and culturing and imaging the mouse urogenital system.
Methods Mol Biol. 2012;886:3-11. doi: 10.1007/978-1-61779-851-1_1.
4
Growth and morphogenesis of embryonic mouse organs on biopore membrane.胚胎小鼠器官在生物孔膜上的生长与形态发生
In Vitro Cell Dev Biol. 1990 Dec;26(12):1119-20. doi: 10.1007/BF02623685.
5
In vitro induction of nephrogenesis in mouse metanephric mesenchyme with lithium introduction and ureteric bud recombination.
Methods Mol Biol. 2012;886:23-30. doi: 10.1007/978-1-61779-851-1_3.
6
Serum-Free Organ Culture of the Embryonic Mouse Ureter.胚胎小鼠输尿管的无血清器官培养
Methods Mol Biol. 2019;1926:31-38. doi: 10.1007/978-1-4939-9021-4_3.
7
Ex vivo whole embryonic kidney culture: a novel method for research in development, regeneration and transplantation.体外全胚胎肾脏培养:一种用于发育、再生和移植研究的新方法。
J Urol. 2008 Jan;179(1):365-70. doi: 10.1016/j.juro.2007.08.092. Epub 2007 Nov 19.
8
Using Ex Vivo Upright Droplet Cultures of Whole Fetal Organs to Study Developmental Processes during Mouse Organogenesis.利用全胎器官的体外直立液滴培养来研究小鼠器官发生过程中的发育过程。
J Vis Exp. 2015 Oct 21(105):e53262. doi: 10.3791/53262.
9
Protocol to study early lung developmental branching in mouse embryos using explant culture.使用组织培养研究小鼠胚胎早期肺部分支发育的方案。
STAR Protoc. 2024 Sep 20;5(3):103198. doi: 10.1016/j.xpro.2024.103198. Epub 2024 Jul 17.
10
Analysis of migration in primary ureteric bud epithelial cells.
Methods Mol Biol. 2012;886:147-55. doi: 10.1007/978-1-61779-851-1_13.

引用本文的文献

1
The Role of BTBD7 in Normal Development and Tumor Progression.BTBD7 在正常发育和肿瘤进展中的作用。
Technol Cancer Res Treat. 2023 Jan-Dec;22:15330338231167732. doi: 10.1177/15330338231167732.
2
The HIF-1α pathway plays a critical role in salivary gland development in ex vivo organ cultures.HIF-1α 通路在体外器官培养中的唾液腺发育中起着关键作用。
FEBS Open Bio. 2022 Feb;12(2):460-469. doi: 10.1002/2211-5463.13351. Epub 2021 Dec 24.
3
Inhibition of Aurora Kinase B activity disrupts development and differentiation of salivary glands.
抑制极光激酶B的活性会破坏唾液腺的发育和分化。
Cell Death Discov. 2021 Jan 18;7(1):16. doi: 10.1038/s41420-020-00393-w.
4
[Acquisition of submandibular gland in mouse embryo and establishment of an in vitro organ culture model].[小鼠胚胎下颌下腺的获取及体外器官培养模型的建立]
Hua Xi Kou Qiang Yi Xue Za Zhi. 2020 Apr 1;38(2):228-232. doi: 10.7518/hxkq.2020.02.020.
5
Preparation of Cells from Embryonic Organs for Single-Cell RNA Sequencing.用于单细胞RNA测序的胚胎器官细胞制备
Curr Protoc Cell Biol. 2019 Jun;83(1):e86. doi: 10.1002/cpcb.86. Epub 2019 Apr 8.
6
Single Molecule RNA FISH (smFISH) in Whole-Mount Mouse Embryonic Organs.全胚胎小鼠器官中的单分子RNA荧光原位杂交(smFISH)
Curr Protoc Cell Biol. 2019 Jun;83(1):e79. doi: 10.1002/cpcb.79. Epub 2018 Nov 5.
7
Par-1b is required for morphogenesis and differentiation of myoepithelial cells during salivary gland development.在唾液腺发育过程中,Par-1b对于肌上皮细胞的形态发生和分化是必需的。
Organogenesis. 2016 Oct;12(4):194-216. doi: 10.1080/15476278.2016.1252887. Epub 2016 Nov 14.
8
Microfluidic platform for the elastic characterization of mouse submandibular glands by atomic force microscopy.利用原子力显微镜对小鼠下颌下腺进行弹性特性分析的微流控平台。
Biosensors (Basel). 2014 Feb 27;4(1):18-27. doi: 10.3390/bios4010018. eCollection 2014 Mar.
9
MYPT1 regulates contractility and microtubule acetylation to modulate integrin adhesions and matrix assembly.肌球蛋白磷酸酶靶向亚基1(MYPT1)调节收缩性和微管乙酰化,以调控整合素黏附及基质组装。
Nat Commun. 2014 Mar 25;5:3510. doi: 10.1038/ncomms4510.
10
Biocompatible tissue scaffold compliance promotes salivary gland morphogenesis and differentiation.生物相容的组织支架顺应性促进唾液腺形态发生和分化。
Tissue Eng Part A. 2014 Jun;20(11-12):1632-42. doi: 10.1089/ten.TEA.2013.0515. Epub 2014 Feb 27.