Lopes Filipa M, Woolf Adrian S
Faculty of Biology Medicine and Health, Division of Cell Matrix Biology and Regenerative Medicine, School of Biological Sciences, University of Manchester, Manchester, UK.
Royal Manchester Children's Hospital, Manchester University NHS Foundation Trust, Manchester Academic Health Science Centre, Manchester, UK.
Methods Mol Biol. 2019;1926:31-38. doi: 10.1007/978-1-4939-9021-4_3.
The ability to explant and then maintain embryonic tissues in organ culture makes it feasible to study the growth and differentiation of whole organs, or parts or combinations of them, in three dimensions. Moreover, the possible effects of biochemical manipulations or mutations can be explored by visualizing a growing organ. The mammalian renal tract comprises the kidney, ureter, and urinary bladder, and the focus of this chapter is organ culture of the embryonic mouse ureter in serum-free defined medium. Over the culture period, rudiments grow in length, smooth muscle differentiates, and the ureters then undergo peristalsis in a proximal to distal direction.
将胚胎组织移出并在器官培养中维持其生长的能力，使得在三维空间中研究整个器官、其部分或组合的生长与分化成为可能。此外，通过观察生长中的器官，可以探索生化操作或突变可能产生的影响。哺乳动物的泌尿道包括肾脏、输尿管和膀胱，本章的重点是在无血清限定培养基中对胚胎小鼠输尿管进行器官培养。在培养期间，输尿管原基会伸长，平滑肌会分化，然后输尿管会从近端向远端进行蠕动。
