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重金属胁迫下凤眼莲(雨久花科)和大薸(天南星科)抗氧化酶活性的变化

Changes in antioxidant enzyme activities in Eichhornia crassipes (Pontederiaceae) and Pistia stratiotes (Araceae) under heavy metal stress.

作者信息

Odjegba V J, Fasidi I O

机构信息

Department of Botany and Microbiology, University of Lagos, Akoka, Yaba, Lagos, Nigeria.

出版信息

Rev Biol Trop. 2007 Sep-Dec;55(3-4):815-23. doi: 10.15517/rbt.v55i3-4.5958.

DOI:10.15517/rbt.v55i3-4.5958
PMID:19086387
Abstract

Whole plants of Eichhornia crassipes and Pistia stratiotes were exposed to various concentrations (0, 0.1, 0.3, 0.5, 1.0, 3.0 and 5.0 mM) of 8 heavy metals (Ag, Cd, Cr, Cu, Hg, Ni, Pb and Zn) hydroponically for 21 days. Spectrometric assays for the total activity of catalase, peroxidase, and superoxide dismutase in the leaves were studied. At the end of the experimental period, data referred to metal treated plants were compared to data of untreated ones (control). Heavy metals increased the activity of catalase, peroxidase and superoxide dismutase in both species and there was differential inducement among metals. Overall, Zn had the least inducement of antioxidant enzymes in both species while Hg had the highest inducement. The increase in antioxidant enzymes in relation to the control plants was more in E. crassipes than P. stratiotes. The results showed that E. crassipes tolerated higher metal concentrations in a greater number of metals than P. stratiotes.

摘要

将凤眼莲和大薸的整株植物水培暴露于8种重金属(银、镉、铬、铜、汞、镍、铅和锌)的各种浓度(0、0.1、0.3、0.5、1.0、3.0和5.0 mM)下21天。研究了叶片中过氧化氢酶、过氧化物酶和超氧化物歧化酶总活性的光谱测定法。在实验期结束时,将金属处理植物的数据与未处理植物(对照)的数据进行比较。重金属增加了两种植物中过氧化氢酶、过氧化物酶和超氧化物歧化酶的活性,并且不同金属之间存在差异诱导作用。总体而言,锌对两种植物中抗氧化酶的诱导作用最小,而汞的诱导作用最大。与对照植物相比,凤眼莲中抗氧化酶的增加比大薸更多。结果表明,与大薸相比,凤眼莲能耐受更多种类金属的更高浓度。

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