Afaghi A, Zare S, Heidari R, Asadpoor Y, Viayeh R Malekzadeh
Department of Biology, Faculty of Science, Urmia University, Urmia, Iran.
Pak J Biol Sci. 2007 May 15;10(10):1655-60. doi: 10.3923/pjbs.2007.1655.1660.
The objective of this study was to evaluate the possible effects of CuSO4 exposure on variations of glucose and cortisol levels in Cyprinus carpio. Three replicates of 6 fish were subjected to two sub-lethal concentrations of CuSO4 (0.16 and 0.53 mg L(-1)) for 14 and 21 days. Blood samples were isolated from the fish following the exposure, to measure the levels of cortisol and glucose compared to the control group. The results showed significant increases (p < 0.05) in cortisol levels for both fish groups after 14 days of exposure, whereas, the levels of blood cortisol in both groups did not differ from that of control when the fish subjected to copper sulfate for 21 days. We found significant increases (p < 0.05) in the levels of blood glucose of two groups of fish after 14 days of exposure to two doses of CuSO4, as well as significant decrease in the blood glucose of both groups exposed for 21 days. In the later treatment, the rate of decrease in group II (exposed to 0.53 mg L(-1) CuSO4) was higher than that of group I (exposed to 0.16 mg L(-1) CuSO4) (p = 0.001 compared to p = 0.032). Our findings attest that exposing to waterborne copper would affect the levels of both cortisol and glucose, as indicators of stress response in Cyprinus carpio.
本研究的目的是评估硫酸铜暴露对鲤鱼葡萄糖和皮质醇水平变化的可能影响。将6条鱼分为三组，分别暴露于两种亚致死浓度的硫酸铜（0.16和0.53毫克/升）中14天和21天。暴露后从鱼体采集血样，与对照组相比测量皮质醇和葡萄糖水平。结果显示，暴露14天后，两组鱼的皮质醇水平均显著升高（p < 0.05）；而暴露21天后，两组鱼的血液皮质醇水平与对照组无差异。我们发现，暴露于两种剂量的硫酸铜14天后，两组鱼的血糖水平显著升高（p < 0.05）；暴露21天后，两组鱼的血糖水平显著降低。在后期处理中，第二组（暴露于0.53毫克/升硫酸铜）的降低速率高于第一组（暴露于0.16毫克/升硫酸铜）（p = 0.001，而第一组p = 0.032）。我们的研究结果证明，暴露于水中的铜会影响鲤鱼作为应激反应指标的皮质醇和葡萄糖水平。
