Gaffarzadeh-Namazi L, Asghari-Zakaria R, Babaeian N, Kazemi-Tabar K
'Demartment of Agronomy, Mazandaran University, Sari, Iran.
Pak J Biol Sci. 2007 Jun 1;10(11):1811-6. doi: 10.3923/pjbs.2007.1811.1816.
Karyotype and C-banding patterns of mitotic chromosomes in 10 cultivars and landraces of lentil were studied. Aceto-iron-hematoxilin staining and Giemsa C-banding techniques were used to staining choromosomes and banding patterns analysis, respectively. Chromosome characteristics including long arm, short arm and chromosome lengths, total length of chromosome set, arm ratio index, relative chromosome length, the width and position of each band, heterochromatin percent per chromosome and per chromosome set were measured using Micromeasure software. The results of this study revealed that the genome of this species consisted of four pairs of metacentric and three pairs of submetacentric chromosomes. Chromosome 4 had a secondary constriction near centromeric region of its long arm. Arm ratio index of chromosomes ranged from 1.24 in chromosome 3 to 2.38 in chromosomes 6. Each chromosome, having a distinctive banding pattern, was recognizable. Karyological characteristics and banding patterns of all materials studied were similar to each other, however, some polymorph C-bands were observed on chromosome arms.
对10个小扁豆品种和地方品种有丝分裂染色体的核型和C带模式进行了研究。分别采用乙酰铁苏木精染色和吉姆萨C带技术对染色体进行染色和带型分析。利用Micromeasure软件测量染色体特征，包括长臂、短臂和染色体长度、染色体组全长、臂比指数、相对染色体长度、每条带的宽度和位置、每条染色体和每个染色体组的异染色质百分比。本研究结果表明，该物种的基因组由四对中着丝粒染色体和三对亚中着丝粒染色体组成。染色体4在其长臂的着丝粒区域附近有一个次缢痕。染色体的臂比指数范围从第3号染色体的1.24到第6号染色体的2.38。每条染色体都有独特的带型，易于识别。所有研究材料的核型特征和带型彼此相似，然而，在染色体臂上观察到一些多态性C带。
