黑种草（Nigella sativa (L.)）种子水提取物对绿豆（Vigna radiata (L.)）发芽和幼苗生长的生物活性。

Biological activity of seed aqueous extract of Nigella sativa (L.) on germination and seedling growth of Vigna radiata (L.).

作者信息

Al-Charchafchi M R, Al-Shuhumi Hkoula, Al-Meselhy Shamsa, Al-Busadi Maya, Al-Shuhumi Haleema

机构信息

Department of Applied Sciences, Higher College of Technology, P.O. Box 74, Postal Code 133, Al-Khuwair, Oman.

出版信息

Pak J Biol Sci. 2007 Dec 1;10(23):4319-22. doi: 10.3923/pjbs.2007.4319.4322.

DOI:10.3923/pjbs.2007.4319.4322

PMID:19086596

Abstract

Seeds aqueous extract of Nigella sativa were evaluated for their effect on germination and seedling growth of Vigna radiata. Two different methods were used to prepare the seed aqueous extract. The aqueous extracts prepared were found to affect germination percentage and seedling growth of V. radiata. The germination percentage and seedling growth of V. radiata decreased as the concentration of the seeds aqueous extracts of N. sativa increased. Severe toxicity was observed at high concentrations and moderate toxicity at low concentrations in comparison with water control. Seeds aqueous extract significantly inhibited root length more than shoot. The aqueous dried powdered extract soaked for 4 days with or without boiling had inhibitory effect on germination and seedling growth of V. radiata, but method 2 (with boiling) was the most effective one specially on root length.

摘要

对黑种草种子水提取物对绿豆发芽和幼苗生长的影响进行了评估。采用两种不同方法制备种子水提取物。结果发现，所制备的水提取物会影响绿豆的发芽率和幼苗生长。随着黑种草种子水提取物浓度的增加，绿豆的发芽率和幼苗生长率降低。与水对照相比，高浓度时观察到严重毒性，低浓度时观察到中等毒性。种子水提取物对根长的抑制作用明显大于对茎的抑制作用。经煮沸或未煮沸浸泡4天的水干燥粉末提取物对绿豆的发芽和幼苗生长有抑制作用，但方法2（煮沸）对根长的抑制效果最为显著。