Saadia Mubshara, Rehman Saba, Robin Sehrish, Ruby Tahira, Sher Muhammad, Siddiqui Waseeq Ahmad, Khan Mahmood Ahmad
Department of Chemistry, University of Sargodha, Sargodha, Pakistan.
Department of Life Sciences, The Islamia University of Bahawalpur, Pakistan.
Pak J Pharm Sci. 2017 Sep;30(5):1679-1690.
Dengue infection is rapidly spreading in most of the countries of south Asia. It is of utmost importance to explore the plants with "anti-thrombocytopenic activity" the dreadful response of dengue fever. The present study was conducted to investigate the potential of aqueous extract of Nigella sativa (black cumin) seeds in alleviating the severity of dengue disease by raising the platelet count (PLT). Serum samples of thirty patients with dengue hemorrhagic fever (DHF) were analysed for different biochemical parameters. When compared with control groups, the patients were found with very low PLT count (7.62 fold), reduced antioxidant levels; catalase (1.4 fold), ascorbic acid (1.1 fold), bilirubin (1.06 fold), and severe deficiency of micronutrient concentrations; cobalt (2.27 fold), iron (2.35 fold) and nickel (71.46 fold). Similar parameters were studied in albino rats to observe the changes in serum levels of biochemical markers, after administration of single dose of choloroquine phosphate (IM, 1.5 mL saline). The drug successfully induced thrombocytopenia along with significant decrease in levels of antioxidants and trace metals. Administration of N. sativa aqueous seed extract (15.25 mg/kg/bw) for 12 days resulted in an increase in PLT count (1.59 fold) as compared to control group. N. sativa post-treatment was found effective in elevating the serum levels of catalase, ascorbic acid, and bilirubin (1.06, 1.58 and 0.4 folds respectively). However, the N. sativa pre-treatment was useful in increasing the levels of micronutrients; iron, nickel and cobalt when compared to quinine-induced group. From the above findings it was suggested that N. sativa seed aqueous extract supplementation would be a promising solution for declined PLT count and associated consequences.
登革热感染正在南亚的大多数国家迅速蔓延。探索具有“抗血小板减少活性”的植物对于应对登革热这种可怕疾病至关重要。本研究旨在通过提高血小板计数（PLT）来调查黑种草籽水提取物减轻登革热疾病严重程度的潜力。对30例登革出血热（DHF）患者的血清样本进行了不同生化参数的分析。与对照组相比，发现患者的血小板计数极低（低7.62倍），抗氧化剂水平降低；过氧化氢酶（低1.4倍）、抗坏血酸（低1.1倍）、胆红素（低1.06倍），以及微量营养素浓度严重缺乏；钴（低2.27倍）、铁（低2.35倍）和镍（低71.46倍）。在白化大鼠中研究了类似参数，以观察单次注射磷酸氯喹（肌肉注射，1.5 mL生理盐水）后生化标志物血清水平的变化。该药物成功诱导了血小板减少，同时抗氧化剂和微量元素水平显著降低。给予黑种草籽水提取物（15.25 mg/kg体重）12天，与对照组相比，血小板计数增加（增加1.59倍）。发现黑种草籽治疗后能有效提高血清过氧化氢酶、抗坏血酸和胆红素水平（分别为1.06、1.58和0.4倍）。然而，与奎宁诱导组相比，黑种草籽预处理有助于提高微量元素铁、镍和钴的水平。从上述研究结果表明，补充黑种草籽水提取物可能是解决血小板计数下降及相关后果的一种有前景的方法。
