Kyaw Win Ohnmar, Uhlig Albrecht, Köller Gábor, Sack Ulrich, Schusser Gerald Fritz
Medizinische Tierklinik, Veterinärmedizinische Fakultät, Universität Leipzig.
Berl Munch Tierarztl Wochenschr. 2008 Nov-Dec;121(11-12):440-5.
A total of 50 adult horses were classified into 4 groups: healthy horses (group 1, n = 14), horses with non-strangulation obstruction (group 2, n = 13), horses with strangulation obstruction (group 3, n = 11) and horses with acute colitis and severe inflammation (peritonitis or thrombophlebitis) (group 4, n = 12). Eleven euthanized horses recreated from group 3 (4 horses with small intestinal strangulation obstruction and hemolytic peritoneal fluid) and group 4 (7 horses with septic peritonitis) were taken as group 5. Free hemoglobin (fr. Hb) and tumor necrosis factor-alpha (TNFa) were measured by method of Kahn and by Duo Set ELISA Development Kit. The concentrations of fr. Hb and TNF-a were significantly higher (p <0.01) in group 3 (medians: 8.0 micromol/l; 14665 pg/ml) and group 4 (9.1; 15081) in comparison to healthy horses (0.61; 573) and group 2 (0.5; 821), whereas group 2 showed no significant difference with group 1.There was a positive correlation between fr. Hb and TNF-alpha (r = 0.82; P = 0.01) in group 4. Concentrations of both fr. Hb and TNF-alpha were significantly (p <0,01) higher in euthanized horses of group 5 than in medically treated horses of group 2.The high concentration of fr. Hb could be a proinflammatory mediator to stimulate high production ofTNF-alpha known as a proinflammatory cytokine. Both of these parameters could contribute to the pathogenesis of systemic inflammatory response syndrome: fever, tachycardia, tachypnoe, leukopenia, thrombocytopenia, and lactic acidosis. Based on this, increased concentrations of both fr. Hb and TNF-alpha could be a guarded prognostic indicator in horses with strangulation obstruction, acute colitis or septic peritonitis.
总共50匹成年马被分为4组：健康马（第1组，n = 14）、非绞窄性梗阻马（第2组，n = 13）、绞窄性梗阻马（第3组，n = 11）以及患有急性结肠炎和严重炎症（腹膜炎或血栓性静脉炎）的马（第4组，n = 12）。从第3组（4匹患有小肠绞窄性梗阻和溶血性腹腔积液的马）和第4组（7匹患有脓毒性腹膜炎的马）选取11匹安乐死的马作为第5组。采用卡恩法和双抗体夹心ELISA试剂盒测定游离血红蛋白（fr. Hb）和肿瘤坏死因子-α（TNFα）。与健康马（中位数：0.61 μmol/l；573 pg/ml）和第2组（0.5；821）相比，第3组（中位数：8.0 μmol/l；14665 pg/ml）和第4组（9.1；15081）的fr. Hb和TNF-α浓度显著更高（p <0.01），而第2组与第1组无显著差异。第4组中fr. Hb与TNF-α之间存在正相关（r = 0.82；P = 0.01）。第5组安乐死马的fr. Hb和TNF-α浓度均显著高于第2组接受药物治疗的马（p <0.01）。高浓度的fr. Hb可能是一种促炎介质，可刺激作为促炎细胞因子的TNF-α的高产生。这两个参数都可能导致全身炎症反应综合征的发病机制：发热、心动过速、呼吸急促、白细胞减少、血小板减少和乳酸酸中毒。基于此，fr. Hb和TNF-α浓度升高可能是绞窄性梗阻、急性结肠炎或脓毒性腹膜炎马匹预后不良的指标。
