Higashi-Okai Kiyoka, Ishida Emi, Nakamura Yumiko, Fujiwara Satomi, Okai Yasuji
Division of Food and Nutrition, Hiroshima Jyogakuin University, Ushita-higashi, Higashi-ku, Hiroshima 732-0063, Japan.
J UOEH. 2008 Dec 1;30(4):375-89. doi: 10.7888/juoeh.30.375.
To estimate the preventive potential of Japanese traditional cereals against oxygen radical-related chronic diseases such as cardiovascular diseases and diabetes, antioxidant and radical-scavenging activities in the extracts of five Japanese traditional cereal grains were analyzed by using an assay system of lipid peroxidation and a radical compound, 1,1-diphenyl-2-picrylhydrazyl (DPPH). DPPH radical-scavenging activities in the extracts of Japanese cereal grains were divided into two groups. One group including Japanese sorghum, black rice and red rice showed strong radical-scavenging activities, but the other group including Japanese barnyard millet and foxtail millet did not exhibit significant radical-scavenging activities. The DPPH radical-scavenging activities of these extracts were closely correlated to the contents of phenolic compound in the extracts, but not to the sugar or protein content in the extracts. In contrast, all the methanol and water extracts of the cereal grains caused significant antioxidant activities against hydroperoxide generation in the peroxidation of linoleic acid, in which the water extracts of these cereal grains caused much higher antioxidant activities than the methanol extracts of the same cereals. These results suggest that Japanese traditional cereals contain qualitatively different principles associated with antioxidant and radical-scavenging activities, and possible principles responsible for the antioxidant and radical-scavenging activities in the cereal grains are discussed.
为评估日本传统谷物对心血管疾病和糖尿病等与氧自由基相关的慢性疾病的预防潜力，利用脂质过氧化检测系统和自由基化合物1,1 - 二苯基 - 2 - 苦基肼（DPPH）分析了五种日本传统谷物提取物中的抗氧化和自由基清除活性。日本谷物提取物中的DPPH自由基清除活性分为两组。一组包括日本高粱、黑米和红米，表现出较强的自由基清除活性，而另一组包括日本稗子和粟，未表现出显著的自由基清除活性。这些提取物的DPPH自由基清除活性与提取物中酚类化合物的含量密切相关，而与提取物中的糖或蛋白质含量无关。相反，谷物的所有甲醇提取物和水提取物对亚油酸过氧化过程中氢过氧化物的产生均具有显著的抗氧化活性，其中这些谷物的水提取物比相同谷物的甲醇提取物具有更高的抗氧化活性。这些结果表明，日本传统谷物含有在抗氧化和自由基清除活性方面性质不同的成分，并对谷物中可能负责抗氧化和自由基清除活性的成分进行了讨论。
