Inaoka Takashi, Satomura Takenori, Fujita Yasutaro, Ochi Kozo
National Food Research Institute, Ibaraki, Japan.
FEMS Microbiol Lett. 2009 Feb;291(2):151-6. doi: 10.1111/j.1574-6968.2008.01450.x. Epub 2008 Dec 11.
Bacillus subtilis GlcP regulates a secondary metabolism, the neotrehalosadiamine synthesis pathway, by repressing a neotrehalosadiamine biosynthesis operon in response to glucose present in the medium. Here, we investigated, by use of transcriptome, additional effects of glcP disruption on other gene expression. In the GlcP-null mutant, the expression of alsSD and maeN was decreased, while the expression of licBCAH, ntdABC, yyaH-maa, and yyaJ was increased. The effect caused by loss of GlcP function was, however, completely negated in a mutant lacking the ability to synthesize neotrehalosadiamine. Moreover, addition of neotrehalosadiamine into the growth medium had no effect on the expression of these genes, indicating that GlcP-promoted regulation was exerted depending on de novo neotrehalosadiamine synthesis rather than neotrehalosadiamine per se. These findings suggest that GlcP participates in regulation of certain genes by repressing the neotrehalosadiamine biosynthesis operon. This novel regulation system may provide new insights into study of B. subtilis gene expression.
枯草芽孢杆菌的GlcP通过响应培养基中存在的葡萄糖抑制新海藻糖二胺生物合成操纵子，从而调节一种次级代谢，即新海藻糖二胺合成途径。在此，我们利用转录组研究了glcP缺失对其他基因表达的额外影响。在GlcP缺失突变体中，alsSD和maeN的表达降低，而licBCAH、ntdABC、yyaH - maa和yyaJ的表达增加。然而，在缺乏合成新海藻糖二胺能力的突变体中，GlcP功能丧失所导致的影响被完全消除。此外，在生长培养基中添加新海藻糖二胺对这些基因的表达没有影响，这表明GlcP促进的调节作用取决于新海藻糖二胺的从头合成，而不是新海藻糖二胺本身。这些发现表明，GlcP通过抑制新海藻糖二胺生物合成操纵子参与某些基因的调节。这种新的调节系统可能为枯草芽孢杆菌基因表达的研究提供新的见解。
