Denoeud France, Aury Jean-Marc, Da Silva Corinne, Noel Benjamin, Rogier Odile, Delledonne Massimo, Morgante Michele, Valle Giorgio, Wincker Patrick, Scarpelli Claude, Jaillon Olivier, Artiguenave François
CEA, DSV, Institut de Génomique, Genoscope, 2 rue Gaston Crémieux, CP5706, 91057 Evry, France.
Genome Biol. 2008;9(12):R175. doi: 10.1186/gb-2008-9-12-r175. Epub 2008 Dec 16.
Next generation technologies enable massive-scale cDNA sequencing (so-called RNA-Seq). Mainly because of the difficulty of aligning short reads on exon-exon junctions, no attempts have been made so far to use RNA-Seq for building gene models de novo, that is, in the absence of a set of known genes and/or splicing events. We present G-Mo.R-Se (Gene Modelling using RNA-Seq), an approach aimed at building gene models directly from RNA-Seq and demonstrate its utility on the grapevine genome.
新一代技术实现了大规模的cDNA测序（即所谓的RNA测序）。主要由于在将短读段比对到外显子-外显子连接区域存在困难，到目前为止尚未有人尝试使用RNA测序从头构建基因模型，即在没有一组已知基因和/或剪接事件的情况下构建基因模型。我们提出了G-Mo.R-Se（利用RNA测序进行基因建模），一种旨在直接从RNA测序构建基因模型的方法，并在葡萄基因组上展示了其效用。
