[盆底器官脱垂女性主韧带中弹性蛋白、赖氨酰氧化酶和弹性蛋白酶的表达]

[Expression of elastin, lysyl oxidase and elafin in the cardinal ligament of women with pelvic organ prolapse].

作者信息

Zhang Shi-Qian, Zhang Lin-Lin, Yu Hao

机构信息

Department of Gynecology, Qilu Hospital of Shandong University, Jinan 250012, China.

出版信息

Zhonghua Fu Chan Ke Za Zhi. 2008 Sep;43(9):675-9.

PMID:19087518

Abstract

OBJECTIVE

To investigate expression of elastin, lysyl oxidase (LOX) and elafin in cardinal ligament of women with pelvic organ prolapse (POP) so as to determine their contributions to POP.

METHODS

The cardinal ligament samples were obtained from 60 POP subjects and 60 non-POP control women undergoing hysterectomy. RT-PCR was used to verify the mRNA level of elastin, LOX and elafin. The protein concentration of the three genes was determined by western blotting technique, electrophoretic separation and quantification.

RESULTS

The premenopausal and postmenopausal POP groups demonstrated significantly decreased expressions of elastin in cardinal ligament both in mRNA and protein levels than control group (mRNA 0.42 +/- 0.22, 0.26 +/- 0.20 versus 0.79 +/- 0.30, 0.63 +/- 0.23; protein 0.44 +/- 0.32, 0.20 +/- 0.19 versus 0.89 +/- 0.27, 0.78 +/- 0.25; P < 0.05). There was an identical tendency in the expression of LOX (mRNA 0.37 +/- 0.18, 0.20 +/- 0.14 versus 0.65 +/- 0.22, 0.53 +/- 0.20; protein 0.45 +/- 0.27, 0.26 +/- 0.21 versus 0.85 +/- 0.39, 0.69 +/- 0.31; P < 0.05). In POP group, the mRNA and protein levels of elastin and LOX in postmenopausal patients were significantly lower than premenopausal patients (P < 0.05). Inversely, POP group demonstrated an increased expression of elafin in cardinal ligament both in mRNA and protein levels than corresponding control group (mRNA 1.33 +/- 0.35, 1.47 +/- 0.37 versus 0.62 +/- 0.25, 0.55 +/- 0.24; protein 0.85 +/- 0.30, 0.76 +/- 0.35 versus 0.21 +/- 0.15, 0.29 +/- 0.22; P < 0.05). There was no significant difference in the expression of elafin between premenopausal and postmenopausal POP groups either in mRNA or protein levels (P > 0.05). There was a positive correlation between elastin and LOX both in mRNA and protein levels in POP group(r = 0.9959, 0.9708; P < 0.05), but there was no correlation between elastin and elafin (r = -0.0402, -0.0365; P > 0.05).

CONCLUSIONS

The results suggest that the decreased expression of elastin and LOX and the increased expression of elafin in the cardinal ligaments may contribute to POP.

摘要

目的

研究盆腔器官脱垂（POP）女性主韧带中弹性蛋白、赖氨酰氧化酶（LOX）和弹性蛋白酶的表达，以确定它们在POP中的作用。

方法

从60例接受子宫切除术的POP患者和60例非POP对照女性中获取主韧带样本。采用逆转录聚合酶链反应（RT-PCR）验证弹性蛋白、LOX和弹性蛋白酶的mRNA水平。通过蛋白质印迹技术、电泳分离和定量分析来测定这三种基因的蛋白质浓度。

结果

绝经前和绝经后POP组主韧带中弹性蛋白的mRNA和蛋白质水平均显著低于对照组（mRNA：0.42±0.22，0.26±0.20对比0.79±0.30，0.63±0.23；蛋白质：0.44±0.32，0.20±0.19对比0.89±0.27，0.78±0.25；P<0.05）。LOX的表达也有相同趋势（mRNA：0.37±0.18，0.20±0.14对比0.65±0.22，0.53±0.20；蛋白质：0.45±0.27，0.26±0.21对比0.85±0.39，0.69±0.31；P<0.05）。在POP组中，绝经后患者弹性蛋白和LOX的mRNA和蛋白质水平显著低于绝经前患者（P<0.05）。相反，POP组主韧带中弹性蛋白酶的mRNA和蛋白质水平均高于相应对照组（mRNA：1.33±0.35，1.47±0.37对比0.62±0.25，0.55±0.24；蛋白质：0.85±0.30，0.76±0.35对比0.21±0.15，0.29±0.22；P<0.05）。绝经前和绝经后POP组之间弹性蛋白酶的mRNA和蛋白质水平均无显著差异（P>0.05）。POP组中弹性蛋白和LOX的mRNA和蛋白质水平均呈正相关（r = 0.9959，0.9708；P<0.05），但弹性蛋白与弹性蛋白酶之间无相关性（r = -0.0402，-0.0365；P>0.05）。