严重盆腔器官脱垂的绝经前女性阴道组织中赖氨氧化酶家族酶的表达
LOX family enzymes expression in vaginal tissue of premenopausal women with severe pelvic organ prolapse.
作者信息
Alarab May, Bortolini Maria A T, Drutz Harold, Lye Stephen, Shynlova Oksana
机构信息
Division of Urogynecology and Reconstructive Pelvic Surgery, Department of Obstetrics and Gynecology, Mount Sinai Hospital, University of Toronto, 700 University Avenue, Suite 3083, Toronto, ON M5G 1Z5, Canada.
出版信息
Int Urogynecol J. 2010 Nov;21(11):1397-404. doi: 10.1007/s00192-010-1199-9. Epub 2010 Jun 18.
INTRODUCTION AND HYPOTHESIS
The extracellular matrix proteins collagen and elastin provide tissue strength and resilience, whereas lysyl oxidase enzymes play a major role in their stabilization. This study examines the expression and tissue localization of lysyl oxidase family proteins in the anterior vaginal wall of premenopausal women with advanced pelvic organ prolapse (POP, n = 15) and asymptomatic controls (n = 11). All women were in the proliferative phase of menstrual cycle.
METHODS
Total mRNAs and proteins extracted from the vaginal tissue were examined by real-time polymerase chain reaction and immunoblotting, and tissue specimens were analyzed by immunohistochemistry.
RESULTS
The expression of LOX, LOXL1, and LOXL3 genes as well as LOX and LOXL3 proteins were significantly reduced in POP patients (P < 0.05). Immunolocalization of LOX family proteins was confirmed in all vaginal specimens.
CONCLUSION
We proposed that reduced expression of LOX enzymes may result in defective assembly of pelvic tissues and development of POP.
引言与假设
细胞外基质蛋白胶原蛋白和弹性蛋白赋予组织强度和弹性，而赖氨酰氧化酶在其稳定过程中起主要作用。本研究检测了晚期盆腔器官脱垂（POP，n = 15）的绝经前女性和无症状对照者（n = 11）阴道前壁中赖氨酰氧化酶家族蛋白的表达及组织定位。所有女性均处于月经周期的增殖期。
方法
通过实时聚合酶链反应和免疫印迹法检测从阴道组织中提取的总mRNA和蛋白质，并通过免疫组织化学分析组织标本。
结果
POP患者中LOX、LOXL1和LOXL3基因以及LOX和LOXL3蛋白的表达显著降低（P < 0.05）。在所有阴道标本中均证实了LOX家族蛋白的免疫定位。
结论
我们认为LOX酶表达降低可能导致盆腔组织组装缺陷和POP的发生。
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