[胃结肠网膜内脂素mRNA表达与妊娠期糖尿病的相关性]

[Correlation between the expression of gastrocolic omentum visfatin mRNA and gestational diabetes mellitus].

作者信息

Liang Yan, Xu Xian-Ming, Wang Hong-Sheng, Wang Pei-Wei

机构信息

Department of Obstetrics and Gynecology, First Affiliated People's Hospital, Jiaotong University, Shanghai 200080, China.

出版信息

Zhonghua Fu Chan Ke Za Zhi. 2008 Nov;43(11):824-7.

PMID:19087564

Abstract

OBJECTIVE

To investigate the relationship between different expression of gastrocolic omentum visfatin(VF) mRNA and gestational diabetes mellitus (GDM) in late trimester pregnancy.

METHODS

One hundred cases of pregnant women in late trimester were divided into two groups, including 45 GDM women (GDM group) and 55 normal glucose tolerance women (NGT group). Semi-quantitative RT-PCR was applied to determine the expression of VF mRNA in gastrocolic omentum. Fast-blood glucose, insulin, cholesterol (TC) and triglyceride (TG) levels were measured. The progestational body mass index (BMI) and the homeostasis model assessment (HOMA) insulin resistance (IR) were calculated in these cases.

RESULTS

The expression of gastrocolic omentum VF mRNA in GDM group and NGT group were 0.8 +/- 0.4, 0.5 +/- 0.3. Fast-blood glucose levels were (4.12 +/- 0.14), (3.65 +/- 0.13) mmol/L. Insulin levels were (72 +/- 5), (61 +/- 5) pmol/L. TG levels were (5.6 +/- 0.3), (3.8 +/- 0.3) mmol/L. TC levels were (5.6 +/- 0.9), (3.9 +/- 0.3) mmol/L. HOMA-IR were 12.5 +/- 5.9, 9.5 +/- 0.8. The progestational BMIs were (22.6 +/- 0.8), (20.9 +/- 0.4) kg/m(2). All the indices in the GDM group were significantly higher than NGT group (P < 0.05). Furthermore, the expression of gastrocolic omentum VF mRNA had a positive correlation with BMI (r = 0.32, P < 0.01), but had no association with insulin resistance or metabolic parameters.

CONCLUSION

The experiment demonstrates that different expression of VF mRNA has a strong correlation with GDM and obesity.

摘要

目的

探讨晚期妊娠胃结肠网膜内脂素（VF）mRNA表达差异与妊娠期糖尿病（GDM）的关系。

方法

将100例晚期妊娠孕妇分为两组，其中45例GDM孕妇为GDM组，55例糖耐量正常孕妇为NGT组。采用半定量逆转录聚合酶链反应（RT-PCR）检测胃结肠网膜中VF mRNA的表达。测定空腹血糖、胰岛素、胆固醇（TC）和甘油三酯（TG）水平。计算孕前体重指数（BMI）及稳态模型评估（HOMA）胰岛素抵抗（IR）。

结果

GDM组和NGT组胃结肠网膜VF mRNA表达分别为0.8±0.4、0.5±0.3。空腹血糖水平分别为（4.12±0.14）、（3.65±0.13）mmol/L。胰岛素水平分别为（72±5）、（61±5）pmol/L。TG水平分别为（5.6±0.3）、（3.8±0.3）mmol/L。TC水平分别为（5.6±0.9）、（3.9±0.3）mmol/L。HOMA-IR分别为12.5±5.9、9.5±0.8。孕前BMI分别为（22.6±0.8）、（20.9±0.4）kg/m²。GDM组各项指标均显著高于NGT组（P<0.05）。此外，胃结肠网膜VF mRNA表达与BMI呈正相关（r=0.32，P<0.01），但与胰岛素抵抗或代谢参数无关。