Li S, Zhang X Y, Chen X T, Fan D M, Zhan S Y, Chen L J, Shu Y H, Zhang J L
Laboratory of Gastroenterology, Xijing Hospital, Fourth Military Medical University, Xi'an.
Chin Med J (Engl). 1991 May;104(5):358-62.
In the present study, an anti-gastric cancer monoclonal antibody, MGb2, was chosen to prepare antibody-mitomycin C (MMC) conjugate with dextran T-40 as intermediary. Twenty molecules of MMC were introduced into each molecule of antibody while the antigen-binding capacity of the antibody was kept well. The conjugate showed selective cytotoxicity upon human gastric cancer cell line SGC-7901. Radioimmunoimaging and biodistribution studies indicated that after conjugation with MMC via dextran T-40 as intermediary, the tumor localization capacity of the antibody was well retained. When tested in nude mice, inoculated with human gastric carcinoma SGC-7901 in bilateral subrenal capsules, intraperitoneal injection of the conjugate daily for 6 days at a dose of 1 mg/kg gave a tumor inhibitory rate of 68.67%, which was far better than that of free MMC or irrelevant conjugate. No synergetic effect was found in regard to the mixture of MGb2 with MMC.
在本研究中，选择一种抗胃癌单克隆抗体MGb2，以葡聚糖T - 40作为中间体来制备抗体 - 丝裂霉素C（MMC）偶联物。在保持抗体抗原结合能力良好的同时，每个抗体分子引入了20个MMC分子。该偶联物对人胃癌细胞系SGC - 7901表现出选择性细胞毒性。放射免疫显像和生物分布研究表明，通过葡聚糖T - 40作为中间体与MMC偶联后，抗体的肿瘤定位能力得到了很好的保留。在双侧肾包膜下接种人胃癌SGC - 7901的裸鼠中进行测试时，以1 mg/kg的剂量每天腹腔注射偶联物，连续6天，肿瘤抑制率为68.67%，这远比游离MMC或无关偶联物要好。未发现MGb2与MMC混合物有协同作用。
