Zhang S Y, Li S, Chen L J, Shu Y H, Zhang X Y, Chen X T
Shanghai Institute of Materia Medica, Chinese Academy of Sciences.
Zhongguo Yao Li Xue Bao. 1990 Nov;11(6):556-60.
A conjugate of an anti-gastric cancer monoclonal antibody and mitomycin C linked by polyaldehyde dextran T-40 (MGb2-PAD-MMC) was prepared. Nude mice inoculated with human gastric cancer (SGC-7901) xenograft in bilateral subrenal capsule were treated ip with the conjugate at a daily dose containing MGb2 22.4 mg/kg and MMC 1 mg/kg for 6 d since 4 h after inoculation. The efficacy of the conjugate was estimated by the reduction of tumor size which calculated by T/C (%) was 32.2%. If MGb2 in the conjugate was replaced by a normal nude mice IgG (NIgG-PAD-MMC) or the nude mice were treated ip with the dose of MMC alone, the tumor T/C (%) were 58 and 87%, respectively. It was statistically significant between MGb2-PAD-MMC and NIgG-PAD-MMC or MMC treatment. When the above mentioned nude mice with SGC-7901 were treated ip with thrice dose of the conjugate (MGb2 67.2 mg/kg and MMC 3 mg/kg) for 6 d, the tumor growth was inhibited completely. Nevertheless, the same dose of MMC was given to the nude mice resulted in toxic appearance included anorexia, weight loss or even death. Furthermore, when the nude mice were treated ip with MGb2-PAD-MMC 24 h after inoculation, no apparent therapeutic effect was seen. In some experiments, nude mice inoculated with another human transplanted gastric tumor (GA II) xenograft treated ip with a conjugate of MGb2 and MMC or daunorubcin (Dau) 1 day after inoculation.(ABSTRACT TRUNCATED AT 250 WORDS)
制备了一种由聚醛葡聚糖T-40连接的抗胃癌单克隆抗体与丝裂霉素C的偶联物（MGb2-PAD-MMC）。将接种人胃癌（SGC-7901）异种移植物的裸鼠双侧肾包膜下接种，自接种后4小时起，每天腹腔注射含MGb2 22.4mg/kg和MMC 1mg/kg的偶联物，共6天。通过计算肿瘤大小的缩小来评估偶联物的疗效，T/C（%）为32.2%。如果将偶联物中的MGb2替换为正常裸鼠IgG（NIgG-PAD-MMC）或仅给裸鼠腹腔注射MMC，肿瘤的T/C（%）分别为58%和87%。MGb2-PAD-MMC与NIgG-PAD-MMC或MMC治疗之间具有统计学意义。当上述接种SGC-7901的裸鼠腹腔注射三次剂量的偶联物（MGb2 67.2mg/kg和MMC 3mg/kg），共6天时，肿瘤生长被完全抑制。然而，给裸鼠注射相同剂量的MMC会导致出现毒性表现，包括厌食、体重减轻甚至死亡。此外，在接种后24小时给裸鼠腹腔注射MGb2-PAD-MMC，未见明显治疗效果。在一些实验中，接种另一种人移植胃癌（GA II）异种移植物的裸鼠在接种后1天腹腔注射MGb2与MMC或柔红霉素（Dau）的偶联物。（摘要截短至250字）
