趋化因子受体-7在口腔鳞状细胞癌中的表达及其与颈部淋巴结转移的关系

[Expression of chemokine receptor-7 in oral squamous cell carcinoma and its relation to cervical lymph node metastasis].

作者信息

Shang Zheng-jun, Liu Ke, Chen Xin-ming

机构信息

Department of Oral and Maxillofacial Surgery, School of Stomatology, Wuhan University, Wuhan 430079, China.

出版信息

Zhonghua Kou Qiang Yi Xue Za Zhi. 2008 Oct;43(10):592-6.

PMID:19087620

Abstract

OBJECTIVE

To investigate the expression of chemokine receptor CCR7 in oral squamous cell carcinoma (OSCC) and its possible role in directional neck lymph node metastasis.

METHODS

The expression of chemokine receptor CCR7 was examined in 85 cases of OSCC, normal oral mucosa, and Tca8113 and adenoid cystic carcinoma (ACC-M) cell lines using immunohistochemistry, RT-PCR and Western blotting. The relationship between the expression and clinicopathological factors of OSCC was analyzed. After treatment with or without CCR7 antibody, in vitro adhesion assay was performed to compare the adhesion of Tca8113 and ACC-M cells to lymph nodes.

RESULTS

CCR7 was expressed in 66% (56/85) of OSCC cases, and there was a significant difference in CCR7 expression between metastasis group (31/39, 79%) and non-metastasis group (25/46, 54%). RT-PCR and Western blotting also confirmed the presence of positive CCR7 expression in OSCC. Tca8113 cells expressing CCR7 showed stronger ability to adhere to lymph nodes as compared to CCR7-negative ACC-M cells and could be actively inhibited by CCR7 antibody.

CONCLUSIONS

CCR7 may play an important role in the development and lymph node metastasis of oral squamous cell carcinoma.

摘要

目的

探讨趋化因子受体CCR7在口腔鳞状细胞癌（OSCC）中的表达及其在颈部淋巴结定向转移中的可能作用。

方法

采用免疫组织化学、逆转录-聚合酶链反应（RT-PCR）及蛋白质免疫印迹法检测85例OSCC组织、正常口腔黏膜、Tca8113细胞系及腺样囊性癌（ACC-M）细胞系中趋化因子受体CCR7的表达情况。分析OSCC组织中CCR7表达与临床病理因素之间的关系。用或不用CCR7抗体处理细胞后，进行体外黏附试验，比较Tca8113细胞和ACC-M细胞与淋巴结的黏附能力。

结果

85例OSCC组织中66%（56/85）表达CCR7，转移组（31/39，79%）与非转移组（25/46，54%）CCR7表达差异有统计学意义。RT-PCR及蛋白质免疫印迹法也证实OSCC组织中有CCR7阳性表达。与CCR7阴性的ACC-M细胞相比，表达CCR7的Tca8113细胞与淋巴结的黏附能力更强，且可被CCR7抗体有效抑制。