Ferran M, Giménez-Arnau A M, Bellosillo B, Pujol R M, Santamaría-Babi L F
Servicio de Dermatología, Hospital del Mar-IMAS/IMIM, Barcelona, España.
Actas Dermosifiliogr. 2008 Nov;99(9):701-7.
Cutaneous lymphocyte antigen (CLA) is expressed by a subgroup of memory T cells that exhibit skin homing and are implicated in cutaneous T-cell-mediated diseases.
Expression of genes associated with psoriasis was analyzed in keratinocytes taken from patients and healthy individuals and cultured under different conditions, including activation using supernatants from CLA(+) T lymphocytes activated with anti-CD3 and anti-CD28 antibodies.
Keratinocytes from psoriasis patients activated by CLA(+)T lymphocytes expressed higher levels of interferon-inducible protein 10, HLA-DR, intercellular cell adhesion molecule 1, and inducible nitric oxide synthase.
Our results suggest that we have developed an in vitro model that will allow analysis of the effector role of CLA(+) T lymphocytes on keratinocytes in psoriasis. This model may allow the identification of genes involved in the pathology of psoriasis through induction by CLA(+) T lymphocytes.
皮肤淋巴细胞抗原（CLA）由一组记忆T细胞表达，这些记忆T细胞表现出皮肤归巢特性，并与皮肤T细胞介导的疾病有关。
分析取自患者和健康个体的角质形成细胞在不同条件下培养时与银屑病相关基因的表达情况，这些条件包括使用抗CD3和抗CD28抗体激活的CLA(+) T淋巴细胞的上清液进行激活。
由CLA(+) T淋巴细胞激活的银屑病患者角质形成细胞表达更高水平的干扰素诱导蛋白10、HLA-DR、细胞间黏附分子1和诱导型一氧化氮合酶。
我们的结果表明，我们建立了一种体外模型，该模型将有助于分析CLA(+) T淋巴细胞在银屑病中对角质形成细胞的效应作用。该模型可能有助于通过CLA(+) T淋巴细胞的诱导来鉴定参与银屑病病理过程的基因。
