Fürbass R, Marahiel M A
Institut für Biochemie und Molekulare Biologie, Berlin, Germany.
FEBS Lett. 1991 Aug 5;287(1-2):153-6. doi: 10.1016/0014-5793(91)80038-5.
The AbrB protein of B. subtilis represses the transcription of various postexponentially expressed genes, such as the antibiotic biosynthesis gene tycA. Recently, we have shown that ArbB binds to the tycA promoter region at two A + T-rich sites; the 'promoter site' (-60 to -35) and the 'leader site' (+169 to +231). In this study we demonstrate that a Ptyc-lacZ fusion missing the leader region is constitutively expressed in wild-type B. subtilis cells and in B. subtilis cells carrying spoOA or abrB mutations. We also show that substitution mutations within the recently reported potential helix-turn-helix DNA binding motif of AbrB did not affect its specific DNA binding ability.
枯草芽孢杆菌的AbrB蛋白可抑制多种指数后期表达基因的转录，如抗生素生物合成基因tycA。最近，我们发现ArbB可在两个富含A+T的位点与tycA启动子区域结合；即“启动子位点”（-60至-35）和“前导序列位点”（+169至+231）。在本研究中，我们证明缺失前导序列区域的Ptyc-lacZ融合基因在野生型枯草芽孢杆菌细胞以及携带spoOA或abrB突变的枯草芽孢杆菌细胞中组成型表达。我们还表明，最近报道的AbrB潜在螺旋-转角-螺旋DNA结合基序内的取代突变并不影响其特异性DNA结合能力。
